Chronic social stress increases levels of preprogalanin mRNA in the rat locus coeruleus

Philip V. Holmes, D. Caroline Blanchard, Robert J. Blanchard, Linda S. Brady, Jacqueline Crawley

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


Galanin is a 29 amino acid neuropeptide that coexists with norepinephrine in approximately 80% of locus coeruleus (LC) neurons in the rat. The effects of chronic, naturalistic stress on preprogalanin mRNA in the LC was studied. The visible burrow system (VBS) served as the stress paradigm. Long-Evans rats (three males and two females) were housed together in the VBS for 13 days. The males established dominance hierarchies during this period. On day 14, preprogalanin mRNA in the LC was significantly elevated in subordinate rats compared to dominant and control rats, as measured by quantitative in situ hybridization. Levels of mRNA were positively correlated with the number of wounds by day 7 and day 14 and negatively correlated with body weight gain by day 14. These results suggest that the neuropeptide galanin may be overexpressed during chronic stress in rats.

Original languageEnglish (US)
Pages (from-to)655-660
Number of pages6
JournalPharmacology, Biochemistry and Behavior
Issue number4
StatePublished - 1995
Externally publishedYes


  • Dominant
  • Galanin
  • In situ hybridization
  • Subordinate
  • Visible burrow system

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Behavioral Neuroscience
  • Biological Psychiatry
  • Pharmacology
  • Toxicology


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