Chronic lyme borreliosis in the laboratory mouse

Stephen W Barthold, Mark S. De Souza, Jeffrey L. Janotka, Abigail L. Smith, David H. Persing

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

C3H/HeJ mice were Inoculated intraperitoneally with 107 uncloned Borrelia burgdorferi at 4 weeks of age and examined on days 30, 90, 180, and 360. Spirochetes were isolated from multiple tissues at all intervals. Joint and heart disease were present in all mice at 30 days and resolved after 90 days. At 180 and 360 days, some mice had mild recurrent joint and heart disease, and most had peripheral segmental periarteritis. The protein electrophoretic migration of 360-day isolates differed from the original inoculum. The experiment was repeated with C3H/HeN and BALB/cByJ mice inoculated intradermally with 104 cloned B. burgdorferi Characterization of infection and disease at 180 and 360 days were similar to those of the first experiment, but spirochetal proteins of isolates from both intervals displayed no protein variation in electrophoretic mobilities. Spirochetes isolated at 360 days were fully pathogenic in naive mice. Sera from infected mice showed an initial immunoglobulin M response, followed by a sustained immunoglobulin G response, involving IgG1, IgG2a, IgG2b and IgG3, with expanding reactivity against multiple antigens over time. These results indicate that immunocompetent mice sustain persistent infections and develop early acute joint and heart lesions that resolve and then recur intermittently.

Original languageEnglish (US)
Pages (from-to)959-971
Number of pages13
JournalAmerican Journal of Pathology
Volume143
Issue number3
StatePublished - Sep 1993
Externally publishedYes

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Lyme Disease
Spirochaetales
Borrelia burgdorferi
Joint Diseases
Immunoglobulin G
Heart Diseases
Proteins
Inbred C3H Mouse
Infection
Immunoglobulin M
Joints
Antigens
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Barthold, S. W., De Souza, M. S., Janotka, J. L., Smith, A. L., & Persing, D. H. (1993). Chronic lyme borreliosis in the laboratory mouse. American Journal of Pathology, 143(3), 959-971.

Chronic lyme borreliosis in the laboratory mouse. / Barthold, Stephen W; De Souza, Mark S.; Janotka, Jeffrey L.; Smith, Abigail L.; Persing, David H.

In: American Journal of Pathology, Vol. 143, No. 3, 09.1993, p. 959-971.

Research output: Contribution to journalArticle

Barthold, SW, De Souza, MS, Janotka, JL, Smith, AL & Persing, DH 1993, 'Chronic lyme borreliosis in the laboratory mouse', American Journal of Pathology, vol. 143, no. 3, pp. 959-971.
Barthold SW, De Souza MS, Janotka JL, Smith AL, Persing DH. Chronic lyme borreliosis in the laboratory mouse. American Journal of Pathology. 1993 Sep;143(3):959-971.
Barthold, Stephen W ; De Souza, Mark S. ; Janotka, Jeffrey L. ; Smith, Abigail L. ; Persing, David H. / Chronic lyme borreliosis in the laboratory mouse. In: American Journal of Pathology. 1993 ; Vol. 143, No. 3. pp. 959-971.
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