Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis

Douglas J. Fort, Enos L. Stover, Philip L. Strong, F. Jay Murray, Carl L Keen

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The aims of this work were as follows: 1) to determine whether a purified diet currently used for studies with rats was acceptable for reproductive studies in frogs; and 2) to determine whether frogs are sensitive to a deficit of boron (B) in the diet. Adult Xenopus laevis were fed a nonpurified beef liver and lung (BLL) diet (310 μg B/kg), a purified diet supplemented with boron (+B; 1850 μg B/kg), or a purified diet low in boron (-B; 45 μg B/kg) for 120 d. Frogs fed the BLL and +B diets produced 11.3 and 12.2% necrotic eggs, respectively. Abnormal gastrulation occurred in <4% of the fertilized eggs in both groups, and 96-h larval survival exceeded 75% in both groups. In contrast, frogs fed the -B diet for 120 d produced a high proportion of necrotic eggs (54%). Fertilized embryos from the -B diet- fed frogs showed a high frequency of abnormal gastrulation (26.8%), and >80% of the embryos died before 96 h of development. Mean embryo cell counts at X. laevis developmental stage 7.5 (mid-blastula) were significantly lower in the -B embryos than in the BLL or +B embryos. BLL and -B embryos grown in low boron culture media had a high frequency of malformations compared with embryos grown in boron-supplemented media. These studies show that a purified diet that has been used in rodent studies was acceptable for reproduction studies in X. laevis. This work also demonstrates that a diet low in boron markedly impairs normal reproductive function in adult X. laevis, and that administration of the low boron diet results in an increase in both incidence and severity of adverse effects. In addition, these studies demonstrate the usefulness of the X. laevis model in nutrition studies.

Original languageEnglish (US)
Pages (from-to)2055-2060
Number of pages6
JournalJournal of Nutrition
Volume129
Issue number11
StatePublished - 1999

Fingerprint

Boron
Xenopus laevis
boron
Reproduction
Diet
embryo (animal)
Embryonic Structures
diet
beef
lungs
Anura
frogs
Lung
liver
Liver
Blastula
Gastrulation
Eggs
Culture Media
Rodentia

Keywords

  • Development
  • Nutritional essentiality
  • Reproduction
  • Xenopus laevis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Fort, D. J., Stover, E. L., Strong, P. L., Murray, F. J., & Keen, C. L. (1999). Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis. Journal of Nutrition, 129(11), 2055-2060.

Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis. / Fort, Douglas J.; Stover, Enos L.; Strong, Philip L.; Murray, F. Jay; Keen, Carl L.

In: Journal of Nutrition, Vol. 129, No. 11, 1999, p. 2055-2060.

Research output: Contribution to journalArticle

Fort, DJ, Stover, EL, Strong, PL, Murray, FJ & Keen, CL 1999, 'Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis', Journal of Nutrition, vol. 129, no. 11, pp. 2055-2060.
Fort, Douglas J. ; Stover, Enos L. ; Strong, Philip L. ; Murray, F. Jay ; Keen, Carl L. / Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis. In: Journal of Nutrition. 1999 ; Vol. 129, No. 11. pp. 2055-2060.
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abstract = "The aims of this work were as follows: 1) to determine whether a purified diet currently used for studies with rats was acceptable for reproductive studies in frogs; and 2) to determine whether frogs are sensitive to a deficit of boron (B) in the diet. Adult Xenopus laevis were fed a nonpurified beef liver and lung (BLL) diet (310 μg B/kg), a purified diet supplemented with boron (+B; 1850 μg B/kg), or a purified diet low in boron (-B; 45 μg B/kg) for 120 d. Frogs fed the BLL and +B diets produced 11.3 and 12.2{\%} necrotic eggs, respectively. Abnormal gastrulation occurred in <4{\%} of the fertilized eggs in both groups, and 96-h larval survival exceeded 75{\%} in both groups. In contrast, frogs fed the -B diet for 120 d produced a high proportion of necrotic eggs (54{\%}). Fertilized embryos from the -B diet- fed frogs showed a high frequency of abnormal gastrulation (26.8{\%}), and >80{\%} of the embryos died before 96 h of development. Mean embryo cell counts at X. laevis developmental stage 7.5 (mid-blastula) were significantly lower in the -B embryos than in the BLL or +B embryos. BLL and -B embryos grown in low boron culture media had a high frequency of malformations compared with embryos grown in boron-supplemented media. These studies show that a purified diet that has been used in rodent studies was acceptable for reproduction studies in X. laevis. This work also demonstrates that a diet low in boron markedly impairs normal reproductive function in adult X. laevis, and that administration of the low boron diet results in an increase in both incidence and severity of adverse effects. In addition, these studies demonstrate the usefulness of the X. laevis model in nutrition studies.",
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