Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish

Ken M. Jeffries, Susanne M. Brander, Monica T. Britton, Nann A. Fangue, Richard E Connon

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Ibuprofen is one of the most commonly detected pharmaceuticals in wastewater effluent; however, the effects of ibuprofen on aquatic organisms are poorly understood. This study presents the transcriptome-wide response of the inland silverside, Menidia beryllina, to chronic exposure to ibuprofen. At the lowest exposure concentration (0.0115 mg/L), we detected a downregulation of many genes involved in skeletal development, aerobic respiration, and immune function. At the highest exposure concentration (1.15 mg/L), we detected increased expression of regulatory genes in the arachidonic acid metabolism pathway and several immune genes involved in an inflammatory response. Additionally, there was differential expression of genes involved in oxidative stress responses and a downregulation of genes involved in osmoregulation. This study provides useful information for monitoring the effects of this common wastewater effluent contaminant in the environment and for the generation of biomarkers of exposure to ibuprofen that may be transferable to other fish species.

Original languageEnglish (US)
Pages (from-to)17397-17413
Number of pages17
JournalEnvironmental Science and Pollution Research
Volume22
Issue number22
DOIs
StatePublished - Nov 1 2015

Keywords

  • Ecotoxicology
  • Gene expression
  • Inland silverside
  • Microarray
  • NSAIDs
  • qPCR
  • Transcriptomics

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Pollution

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