Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish

Ken M. Jeffries, Susanne M. Brander, Monica T. Britton, Nann A. Fangue, Richard E Connon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Ibuprofen is one of the most commonly detected pharmaceuticals in wastewater effluent; however, the effects of ibuprofen on aquatic organisms are poorly understood. This study presents the transcriptome-wide response of the inland silverside, Menidia beryllina, to chronic exposure to ibuprofen. At the lowest exposure concentration (0.0115 mg/L), we detected a downregulation of many genes involved in skeletal development, aerobic respiration, and immune function. At the highest exposure concentration (1.15 mg/L), we detected increased expression of regulatory genes in the arachidonic acid metabolism pathway and several immune genes involved in an inflammatory response. Additionally, there was differential expression of genes involved in oxidative stress responses and a downregulation of genes involved in osmoregulation. This study provides useful information for monitoring the effects of this common wastewater effluent contaminant in the environment and for the generation of biomarkers of exposure to ibuprofen that may be transferable to other fish species.

Original languageEnglish (US)
Pages (from-to)17397-17413
Number of pages17
JournalEnvironmental Science and Pollution Research
Volume22
Issue number22
DOIs
StatePublished - Nov 1 2015

Fingerprint

Ibuprofen
Fish
Fishes
Genes
gene
fish
Waste Water
Down-Regulation
Effluents
Osmoregulation
Wastewater
Aquatic Organisms
effluent
Aquatic organisms
Regulator Genes
wastewater
osmoregulation
Transcriptome
Arachidonic Acid
Oxidative stress

Keywords

  • Ecotoxicology
  • Gene expression
  • Inland silverside
  • Microarray
  • NSAIDs
  • qPCR
  • Transcriptomics

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Pollution

Cite this

Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish. / Jeffries, Ken M.; Brander, Susanne M.; Britton, Monica T.; Fangue, Nann A.; Connon, Richard E.

In: Environmental Science and Pollution Research, Vol. 22, No. 22, 01.11.2015, p. 17397-17413.

Research output: Contribution to journalArticle

Jeffries, Ken M. ; Brander, Susanne M. ; Britton, Monica T. ; Fangue, Nann A. ; Connon, Richard E. / Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish. In: Environmental Science and Pollution Research. 2015 ; Vol. 22, No. 22. pp. 17397-17413.
@article{baf462edd75843479950bedc946495cb,
title = "Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish",
abstract = "Ibuprofen is one of the most commonly detected pharmaceuticals in wastewater effluent; however, the effects of ibuprofen on aquatic organisms are poorly understood. This study presents the transcriptome-wide response of the inland silverside, Menidia beryllina, to chronic exposure to ibuprofen. At the lowest exposure concentration (0.0115 mg/L), we detected a downregulation of many genes involved in skeletal development, aerobic respiration, and immune function. At the highest exposure concentration (1.15 mg/L), we detected increased expression of regulatory genes in the arachidonic acid metabolism pathway and several immune genes involved in an inflammatory response. Additionally, there was differential expression of genes involved in oxidative stress responses and a downregulation of genes involved in osmoregulation. This study provides useful information for monitoring the effects of this common wastewater effluent contaminant in the environment and for the generation of biomarkers of exposure to ibuprofen that may be transferable to other fish species.",
keywords = "Ecotoxicology, Gene expression, Inland silverside, Microarray, NSAIDs, qPCR, Transcriptomics",
author = "Jeffries, {Ken M.} and Brander, {Susanne M.} and Britton, {Monica T.} and Fangue, {Nann A.} and Connon, {Richard E}",
year = "2015",
month = "11",
day = "1",
doi = "10.1007/s11356-015-4227-y",
language = "English (US)",
volume = "22",
pages = "17397--17413",
journal = "Environmental Science and Pollution Research",
issn = "0944-1344",
publisher = "Springer Science + Business Media",
number = "22",

}

TY - JOUR

T1 - Chronic exposures to low and high concentrations of ibuprofen elicit different gene response patterns in a euryhaline fish

AU - Jeffries, Ken M.

AU - Brander, Susanne M.

AU - Britton, Monica T.

AU - Fangue, Nann A.

AU - Connon, Richard E

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Ibuprofen is one of the most commonly detected pharmaceuticals in wastewater effluent; however, the effects of ibuprofen on aquatic organisms are poorly understood. This study presents the transcriptome-wide response of the inland silverside, Menidia beryllina, to chronic exposure to ibuprofen. At the lowest exposure concentration (0.0115 mg/L), we detected a downregulation of many genes involved in skeletal development, aerobic respiration, and immune function. At the highest exposure concentration (1.15 mg/L), we detected increased expression of regulatory genes in the arachidonic acid metabolism pathway and several immune genes involved in an inflammatory response. Additionally, there was differential expression of genes involved in oxidative stress responses and a downregulation of genes involved in osmoregulation. This study provides useful information for monitoring the effects of this common wastewater effluent contaminant in the environment and for the generation of biomarkers of exposure to ibuprofen that may be transferable to other fish species.

AB - Ibuprofen is one of the most commonly detected pharmaceuticals in wastewater effluent; however, the effects of ibuprofen on aquatic organisms are poorly understood. This study presents the transcriptome-wide response of the inland silverside, Menidia beryllina, to chronic exposure to ibuprofen. At the lowest exposure concentration (0.0115 mg/L), we detected a downregulation of many genes involved in skeletal development, aerobic respiration, and immune function. At the highest exposure concentration (1.15 mg/L), we detected increased expression of regulatory genes in the arachidonic acid metabolism pathway and several immune genes involved in an inflammatory response. Additionally, there was differential expression of genes involved in oxidative stress responses and a downregulation of genes involved in osmoregulation. This study provides useful information for monitoring the effects of this common wastewater effluent contaminant in the environment and for the generation of biomarkers of exposure to ibuprofen that may be transferable to other fish species.

KW - Ecotoxicology

KW - Gene expression

KW - Inland silverside

KW - Microarray

KW - NSAIDs

KW - qPCR

KW - Transcriptomics

UR - http://www.scopus.com/inward/record.url?scp=84947613752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947613752&partnerID=8YFLogxK

U2 - 10.1007/s11356-015-4227-y

DO - 10.1007/s11356-015-4227-y

M3 - Article

C2 - 25731088

AN - SCOPUS:84947613752

VL - 22

SP - 17397

EP - 17413

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

SN - 0944-1344

IS - 22

ER -