Chronic exposure to Low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons

Claire B. de La Serre, Guillaume de Lartigue, Helen E Raybould

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Bacterially derived factors are implicated in the causation and persistence of obesity. Ingestion of a high fat diet in rodents and obesity in human subjects is associated with chronic elevation of low plasma levels of lipopolysaccharide (LPS), a breakdown product of Gram-negative bacteria. The terminals of vagal afferent neurons are positioned within the gut mucosa to convey information from the gut to the brain to regulate food intake and are responsive to LPS. We hypothesized that chronic elevation of LPS could alter vagal afferent signaling. We surgically implanted osmotic mini-pumps that delivered a constant, low-dose of LPS into the intraperitoneal cavity of rats (12.5. μg/kg/hr for 6. weeks). LPS-treated rats developed hyperphagia and showed marked changes in vagal afferent neuron function. Chronic LPS treatment reduced vagal afferent leptin signaling, characterized by a decrease in leptin-induced STAT3 phosphorylation. In addition, LPS treatment decreased cholecystokinin-induced satiety. There was no alteration in leptin signaling in the hypothalamus. These findings offer a mechanism by which a change in gut microflora can promote hyperphagia, possibly leading to obesity.

Original languageEnglish (US)
Pages (from-to)188-194
Number of pages7
JournalPhysiology and Behavior
Volume139
DOIs
StatePublished - Feb 1 2015

Fingerprint

Afferent Neurons
Leptin
Lipopolysaccharides
Hyperphagia
Obesity
Eating
Cholecystokinin
High Fat Diet
Dose
Neuron
Gram-Negative Bacteria
Causality
Hypothalamus
Rodentia
Mucous Membrane
Phosphorylation
Elevation
Rat
Brain

Keywords

  • Metabolic endotoxemia
  • Suppressor of cytokine signaling 3
  • Toll-like receptor 4
  • Vagal afferent neurons

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology
  • Philosophy

Cite this

Chronic exposure to Low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons. / de La Serre, Claire B.; de Lartigue, Guillaume; Raybould, Helen E.

In: Physiology and Behavior, Vol. 139, 01.02.2015, p. 188-194.

Research output: Contribution to journalArticle

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