Chronic consumption of a flavanol- and procyanindin-rich diet is associated with reduced levels of 8-hydroxy-2′-deoxyguanosine in rat testes

Timothy J. Orozco, Janice F. Wang, Carl L Keen

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Cocoa can contain a high concentration of flavanols and procyanidins which have been reported to have strong antioxidative activity. In the present study, male Sprague-Dawley rats were fed diets containing 0, 0.5, 1, or 2% cocoa rich in flavanols for two weeks. Blood, liver, heart and testes were collected and analyzed for markers of oxidative damage. Plasma epicatechin concentrations, 8-hydroxy-2′-deoxyguanosine (8OH2′dG), and oxidized and reduced glutathione were quantitated by HPLC with electrochemical detection. Plasma F2-isoprostanes were measured using an enzyme immunoassay. Plasma epicatechin concentrations increased in a dose-dependant fashion according to the amount of cocoa in the diet (128 nM-790 nM). Cocoa supplementation was associated with lower than normal concentrations of 8OH2′dG in the testes (0.590 + 0.40 vs. 0.328 + 0.29; p < 0.05). Liver and heart 8OH2′dG levels were unaffected by dietary treatment. In erythrocytes, the glutathione pool was significantly less oxidized in the cocoa fed group compared to controls (p < 0.05). In liver and testes, no differences in superoxide dismutase activities were detected. Concentrations of plasma F2-isoprostanes and thiobarbituric acid reactive substances were similar in all groups. These results support the concept that a diet rich in flavanols and procyanidins can improve oxidant defense and reduce tissue markers for oxidative stress, although these effects can be tissue specific.

Original languageEnglish (US)
Pages (from-to)104-110
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2003

Keywords

  • 8-hydroxy-2′-deoxyguanosine
  • Cocoa
  • Epicatechin
  • Flavanols
  • Flavonoids
  • Oxidative damage

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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