Abstract
Cocoa can contain a high concentration of flavanols and procyanidins which have been reported to have strong antioxidative activity. In the present study, male Sprague-Dawley rats were fed diets containing 0, 0.5, 1, or 2% cocoa rich in flavanols for two weeks. Blood, liver, heart and testes were collected and analyzed for markers of oxidative damage. Plasma epicatechin concentrations, 8-hydroxy-2′-deoxyguanosine (8OH2′dG), and oxidized and reduced glutathione were quantitated by HPLC with electrochemical detection. Plasma F2-isoprostanes were measured using an enzyme immunoassay. Plasma epicatechin concentrations increased in a dose-dependant fashion according to the amount of cocoa in the diet (128 nM-790 nM). Cocoa supplementation was associated with lower than normal concentrations of 8OH2′dG in the testes (0.590 + 0.40 vs. 0.328 + 0.29; p < 0.05). Liver and heart 8OH2′dG levels were unaffected by dietary treatment. In erythrocytes, the glutathione pool was significantly less oxidized in the cocoa fed group compared to controls (p < 0.05). In liver and testes, no differences in superoxide dismutase activities were detected. Concentrations of plasma F2-isoprostanes and thiobarbituric acid reactive substances were similar in all groups. These results support the concept that a diet rich in flavanols and procyanidins can improve oxidant defense and reduce tissue markers for oxidative stress, although these effects can be tissue specific.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 104-110 |
| Number of pages | 7 |
| Journal | Journal of Nutritional Biochemistry |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2003 |
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Keywords
- 8-hydroxy-2′-deoxyguanosine
- Cocoa
- Epicatechin
- Flavanols
- Flavonoids
- Oxidative damage
ASJC Scopus subject areas
- Biochemistry
- Endocrinology, Diabetes and Metabolism
Cite this
Chronic consumption of a flavanol- and procyanindin-rich diet is associated with reduced levels of 8-hydroxy-2′-deoxyguanosine in rat testes. / Orozco, Timothy J.; Wang, Janice F.; Keen, Carl L.
In: Journal of Nutritional Biochemistry, Vol. 14, No. 2, 01.02.2003, p. 104-110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chronic consumption of a flavanol- and procyanindin-rich diet is associated with reduced levels of 8-hydroxy-2′-deoxyguanosine in rat testes
AU - Orozco, Timothy J.
AU - Wang, Janice F.
AU - Keen, Carl L
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Cocoa can contain a high concentration of flavanols and procyanidins which have been reported to have strong antioxidative activity. In the present study, male Sprague-Dawley rats were fed diets containing 0, 0.5, 1, or 2% cocoa rich in flavanols for two weeks. Blood, liver, heart and testes were collected and analyzed for markers of oxidative damage. Plasma epicatechin concentrations, 8-hydroxy-2′-deoxyguanosine (8OH2′dG), and oxidized and reduced glutathione were quantitated by HPLC with electrochemical detection. Plasma F2-isoprostanes were measured using an enzyme immunoassay. Plasma epicatechin concentrations increased in a dose-dependant fashion according to the amount of cocoa in the diet (128 nM-790 nM). Cocoa supplementation was associated with lower than normal concentrations of 8OH2′dG in the testes (0.590 + 0.40 vs. 0.328 + 0.29; p < 0.05). Liver and heart 8OH2′dG levels were unaffected by dietary treatment. In erythrocytes, the glutathione pool was significantly less oxidized in the cocoa fed group compared to controls (p < 0.05). In liver and testes, no differences in superoxide dismutase activities were detected. Concentrations of plasma F2-isoprostanes and thiobarbituric acid reactive substances were similar in all groups. These results support the concept that a diet rich in flavanols and procyanidins can improve oxidant defense and reduce tissue markers for oxidative stress, although these effects can be tissue specific.
AB - Cocoa can contain a high concentration of flavanols and procyanidins which have been reported to have strong antioxidative activity. In the present study, male Sprague-Dawley rats were fed diets containing 0, 0.5, 1, or 2% cocoa rich in flavanols for two weeks. Blood, liver, heart and testes were collected and analyzed for markers of oxidative damage. Plasma epicatechin concentrations, 8-hydroxy-2′-deoxyguanosine (8OH2′dG), and oxidized and reduced glutathione were quantitated by HPLC with electrochemical detection. Plasma F2-isoprostanes were measured using an enzyme immunoassay. Plasma epicatechin concentrations increased in a dose-dependant fashion according to the amount of cocoa in the diet (128 nM-790 nM). Cocoa supplementation was associated with lower than normal concentrations of 8OH2′dG in the testes (0.590 + 0.40 vs. 0.328 + 0.29; p < 0.05). Liver and heart 8OH2′dG levels were unaffected by dietary treatment. In erythrocytes, the glutathione pool was significantly less oxidized in the cocoa fed group compared to controls (p < 0.05). In liver and testes, no differences in superoxide dismutase activities were detected. Concentrations of plasma F2-isoprostanes and thiobarbituric acid reactive substances were similar in all groups. These results support the concept that a diet rich in flavanols and procyanidins can improve oxidant defense and reduce tissue markers for oxidative stress, although these effects can be tissue specific.
KW - 8-hydroxy-2′-deoxyguanosine
KW - Cocoa
KW - Epicatechin
KW - Flavanols
KW - Flavonoids
KW - Oxidative damage
UR - http://www.scopus.com/inward/record.url?scp=0037298728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037298728&partnerID=8YFLogxK
U2 - 10.1016/S0955-2863(02)00273-5
DO - 10.1016/S0955-2863(02)00273-5
M3 - Article
C2 - 12667602
AN - SCOPUS:0037298728
VL - 14
SP - 104
EP - 110
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
IS - 2
ER -