Chronic aeroallergen during infancy enhances eotaxin-3 expression in airway epithelium and nerves

Debbie L. Chou, Bruce L. Daugherty, Erin K. McKenna, Willy M. Hsu, Nancy K. Tyler, Charles Plopper, Dallas M. Hyde, Edward S Schelegle, Laurel J Gershwin, Lisa Miller

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have documented that exposure of rhesus monkeys to house dust mite aeroallergen during postnatal development resulted in significant recruitment of eosinophils into the airway mucosa (Clin Exp Allergy 33:1686-1694, 2003). Because eosinophils were not uniformly distributed throughout the five conducting airway generations examined, we speculated that trafficking within anatomic microenvironments of the lung is mediated by differential chemokine expression. To address this question, we used quantitative real-time RT-PCR to evaluate the related eosinophilic chemokines, eotaxin (CCL11), eotaxin-2 (CCL24), and eotaxin-3 (CCL26) within isolated airways of infant monkey lung. Overall, chemokine mRNA expression levels in house dust mite-exposed airways were as follows: eotaxin-3 > eotaxin > eotaxin-2. Immunofluorescence staining for eotaxin-3 and CC chemokine receptor 3 (CCR3) showed positive cells within epithelium and peripherally located nerve fiber bundles of the airway wall. Epithelial volume of eotaxin-3 within the trachea correlated with epithelial volume of major basic protein. CCR3+ and MHC Class II+ dendritic cells, but not eosinophils or mast cells, co-localized within eotaxin-3+ nerve fiber bundles. We conclude that localized expression of eotaxin-3 plays an important role in the recruitment of diverse CCR3+ cell populations to different anatomic microenvironments within the infant airway in response to chronic allergen exposure.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume33
Issue number1
DOIs
StatePublished - Jul 2005

Fingerprint

CCR3 Receptors
Chemokine CCL24
Eosinophils
Pyroglyphidae
Epithelium
Nerve Fibers
Chemokines
Chemokine CCL11
Lung
Trachea
Macaca mulatta
Mast Cells
Allergens
Dendritic Cells
Haplorhini
Fluorescent Antibody Technique
Dust
Real-Time Polymerase Chain Reaction
Hypersensitivity
Mucous Membrane

Keywords

  • Chemokine
  • Development
  • Eosinophil
  • Lung

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Chronic aeroallergen during infancy enhances eotaxin-3 expression in airway epithelium and nerves. / Chou, Debbie L.; Daugherty, Bruce L.; McKenna, Erin K.; Hsu, Willy M.; Tyler, Nancy K.; Plopper, Charles; Hyde, Dallas M.; Schelegle, Edward S; Gershwin, Laurel J; Miller, Lisa.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 33, No. 1, 07.2005, p. 1-8.

Research output: Contribution to journalArticle

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