Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake

Daniel K. Rozelle, Scott D. Hansen, Kenneth B. Kaplan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

During mitosis, chromosome passenger complexes (CPCs) exhibit a well-conserved association with the anaphase spindle and have been implicated in spindle stability. However, their precise effect on the spindle is not clear. In this paper, we show, in budding yeast, that a CPC consisting of CBF3, Bir1, and Sli15, but not Ipl1, is required for normal spindle elongation. CPC mutants slow spindle elongation through the action of the bipolar kinesins Cin8 and Kip1. The same CPC mutants that slow spindle elongation also result in the enrichment of Cin8 and Kip1 at the spindle midzone. Together, these findings argue that CPCs function to organize the spindle midzone and potentially switch motors between force generators and molecular brakes. We also find that slowing spindle elongation delays the mitotic exit network (MEN)-dependent release of Cdc14, thus delaying spindle breakdown until a minimal spindle size is reached. We propose that these CPC- and MEN-dependent mechanisms are important for coordinating chromosome segregation with spindle breakdown and mitotic exit.

Original languageEnglish (US)
Pages (from-to)285-294
Number of pages10
JournalJournal of Cell Biology
Volume193
Issue number2
DOIs
StatePublished - Apr 18 2011

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Kinesin
Anaphase
Chromosomes
Chromosome Segregation
Spindle Apparatus
Saccharomycetales
Mitosis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake. / Rozelle, Daniel K.; Hansen, Scott D.; Kaplan, Kenneth B.

In: Journal of Cell Biology, Vol. 193, No. 2, 18.04.2011, p. 285-294.

Research output: Contribution to journalArticle

Rozelle, Daniel K. ; Hansen, Scott D. ; Kaplan, Kenneth B. / Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake. In: Journal of Cell Biology. 2011 ; Vol. 193, No. 2. pp. 285-294.
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