Chromosomal mapping of the human (MACS) and mouse (Macs) genes encoding the MARCKS protein

Perry J. Blackshear, Jane S. Tuttle, Rebecca J. Oakey, Michael F Seldin, Michèle Chery, Christophe Philippe, Deborah J. Stumpo

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The myristoylated, alanine-rich C-kinase substrate, or MARCKS protein, is a major cellular substrate for protein kinase C that is also a high-affinity calmodulin-binding protein. In addition, it is the prototype of a small family of myristoylated, calmodulin-binding protein kinase C substrate proteins. We isolated a phage clone from a mouse genomic library that spanned the entire coding sequence of the mouse MARCKS protein. The first 612 bp of the putative promoter was 89% identical to a corresponding region of the human promoter, and contained at least 59 potential transcription factor binding sites in analogous locations; both human and mouse promoters lacked TATA boxes. The mouse genomic probe was used to localize the mouse gene to chromosome 10, in the middle of a linkage group that corresponds to a region on human chromosome 6q. These data strongly suggested that the human gene would localize to 6q21. This was confirmed by studies of DNA from a patient with del(6)(q21), in which expression of the human gene encoding MARCKS, MACS, was only about 50% of normal; MARCKS mRNA expression in lymphoblast RNA from this patient was only 22% of normal. These studies confirm that the mouse and human MARCKS proteins are products of the same genes in their respective species; differences in their primary sequence can therefore be attributed to species variation rather than to the existence of related genes.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalGenomics
Volume14
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Blackshear, P. J., Tuttle, J. S., Oakey, R. J., Seldin, M. F., Chery, M., Philippe, C., & Stumpo, D. J. (1992). Chromosomal mapping of the human (MACS) and mouse (Macs) genes encoding the MARCKS protein. Genomics, 14(1), 168-174. https://doi.org/10.1016/S0888-7543(05)80300-3