Abstract
Objective: To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design: Prospective rhesus monkey IVF study. Setting: Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s): Young rhesus macaque females. Intervention(s): In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s): Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s): Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s): These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1230-1237 |
| Number of pages | 8 |
| Journal | Fertility and Sterility |
| Volume | 91 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2009 |
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Keywords
- Aneuploidy
- ART
- cytogenetics
- embryo
- FISH
- IVF
- nonhuman primate
- preimplantation genetic diagnosis
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Chromosomal instability in rhesus macaque preimplantation embryos. / Dupont, Cathérine; Froenicke, Lutz; Lyons, Leslie A; Bavister, Barry D.; Brenner, Carol A.
In: Fertility and Sterility, Vol. 91, No. 4, 04.2009, p. 1230-1237.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chromosomal instability in rhesus macaque preimplantation embryos
AU - Dupont, Cathérine
AU - Froenicke, Lutz
AU - Lyons, Leslie A
AU - Bavister, Barry D.
AU - Brenner, Carol A.
PY - 2009/4
Y1 - 2009/4
N2 - Objective: To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design: Prospective rhesus monkey IVF study. Setting: Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s): Young rhesus macaque females. Intervention(s): In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s): Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s): Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s): These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.
AB - Objective: To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design: Prospective rhesus monkey IVF study. Setting: Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s): Young rhesus macaque females. Intervention(s): In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s): Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s): Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s): These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.
KW - Aneuploidy
KW - ART
KW - cytogenetics
KW - embryo
KW - FISH
KW - IVF
KW - nonhuman primate
KW - preimplantation genetic diagnosis
UR - http://www.scopus.com/inward/record.url?scp=63149148016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63149148016&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2008.01.075
DO - 10.1016/j.fertnstert.2008.01.075
M3 - Article
C2 - 18440514
AN - SCOPUS:63149148016
VL - 91
SP - 1230
EP - 1237
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -