Chromosomal instability in rhesus macaque preimplantation embryos

Cathérine Dupont, Lutz Froenicke, Leslie A Lyons, Barry D. Bavister, Carol A. Brenner

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design: Prospective rhesus monkey IVF study. Setting: Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s): Young rhesus macaque females. Intervention(s): In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s): Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s): Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s): These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.

Original languageEnglish (US)
Pages (from-to)1230-1237
Number of pages8
JournalFertility and Sterility
Volume91
Issue number4
DOIs
StatePublished - Apr 2009

Fingerprint

Chromosomal Instability
Blastocyst
Macaca mulatta
Embryonic Structures
Chromosome Aberrations
Primates
Human Artificial Chromosomes
Animal Models
Bacterial Artificial Chromosomes
Chromosomes, Human, Pair 16
Triploidy
Chromosomes, Human, Pair 13
Haploidy
Human Chromosomes
Aneuploidy
Fluorescence In Situ Hybridization
Research
Oocytes
Color
Chromosomes

Keywords

  • Aneuploidy
  • ART
  • cytogenetics
  • embryo
  • FISH
  • IVF
  • nonhuman primate
  • preimplantation genetic diagnosis

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Chromosomal instability in rhesus macaque preimplantation embryos. / Dupont, Cathérine; Froenicke, Lutz; Lyons, Leslie A; Bavister, Barry D.; Brenner, Carol A.

In: Fertility and Sterility, Vol. 91, No. 4, 04.2009, p. 1230-1237.

Research output: Contribution to journalArticle

Dupont, Cathérine ; Froenicke, Lutz ; Lyons, Leslie A ; Bavister, Barry D. ; Brenner, Carol A. / Chromosomal instability in rhesus macaque preimplantation embryos. In: Fertility and Sterility. 2009 ; Vol. 91, No. 4. pp. 1230-1237.
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