Abstract
Objective: To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design: Prospective rhesus monkey IVF study. Setting: Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s): Young rhesus macaque females. Intervention(s): In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s): Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s): Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s): These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.
Original language | English (US) |
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Pages (from-to) | 1230-1237 |
Number of pages | 8 |
Journal | Fertility and Sterility |
Volume | 91 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2009 |
Keywords
- Aneuploidy
- ART
- cytogenetics
- embryo
- FISH
- IVF
- nonhuman primate
- preimplantation genetic diagnosis
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine