TY - JOUR
T1 - Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle
AU - Nannas, Natalie J.
AU - O'Toole, Eileen T.
AU - Winey, Mark
AU - Murray, Andrew W.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The length of the mitotic spindle varies among different cell types. A simple model for spindle length regulation requires balancing two forces: pulling, due to micro?tubules that attach to the chromosomes at their kinetochores, and pushing, due to interac? tions between microtubules that emanate from opposite spindle poles. In the budding yeast Saccharomyces cerevisiae, we show that spindle length scales with kinetochore number, in? creasing when kinetochores are inactivated and shortening on addition of synthetic or natural kinetochores, showing that kinetochore-microtubule interactions generate an inward force to balance forces that elongate the spindle. Electron microscopy shows that manipulating kinetochore number alters the number of spindle microtubules: adding extra kinetochores increases the number of spindle microtubules, suggesting kinetochore-based regulation of microtubule number.
AB - The length of the mitotic spindle varies among different cell types. A simple model for spindle length regulation requires balancing two forces: pulling, due to micro?tubules that attach to the chromosomes at their kinetochores, and pushing, due to interac? tions between microtubules that emanate from opposite spindle poles. In the budding yeast Saccharomyces cerevisiae, we show that spindle length scales with kinetochore number, in? creasing when kinetochores are inactivated and shortening on addition of synthetic or natural kinetochores, showing that kinetochore-microtubule interactions generate an inward force to balance forces that elongate the spindle. Electron microscopy shows that manipulating kinetochore number alters the number of spindle microtubules: adding extra kinetochores increases the number of spindle microtubules, suggesting kinetochore-based regulation of microtubule number.
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U2 - 10.1091/mbc.E14-01-0016
DO - 10.1091/mbc.E14-01-0016
M3 - Article
C2 - 25318669
AN - SCOPUS:84919493925
VL - 25
SP - 4034
EP - 4048
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 25
ER -