Chromatin immunoprecipitation assays for Myc and N-Myc

Bonnie L. Barrilleaux, Rebecca Cotterman, Paul S Knoepfler

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations


Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip, and, more recently, ChIP-seq to analyze the binding and genomic location of Myc. In this chapter, we describe an effective ChIP protocol using specific validated antibodies to Myc and N-Myc. We discuss the application of this protocol to several types of stem and cancer cells, with a focus on aspects of sample preparation prior to library preparation that are critical for successful Myc ChIP assays. Key variables are discussed and include the starting quantity of cells or tissue, lysis and sonication conditions, the quantity and quality of antibody used, and the identification of reliable target genes for ChIP validation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
ISBN (Print)9781627034289
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • ChIP-seq
  • Chromatin immunoprecipitation
  • Epigenetics
  • Histone modifications
  • Myc
  • N-Myc

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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