Chromatin immunoprecipitation assays for Myc and N-Myc

Bonnie L. Barrilleaux, Rebecca Cotterman, Paul S Knoepfler

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip, and, more recently, ChIP-seq to analyze the binding and genomic location of Myc. In this chapter, we describe an effective ChIP protocol using specific validated antibodies to Myc and N-Myc. We discuss the application of this protocol to several types of stem and cancer cells, with a focus on aspects of sample preparation prior to library preparation that are critical for successful Myc ChIP assays. Key variables are discussed and include the starting quantity of cells or tissue, lysis and sonication conditions, the quantity and quality of antibody used, and the identification of reliable target genes for ChIP validation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages117-133
Number of pages17
Volume1012
ISBN (Print)9781627034289
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1012
ISSN (Print)10643745

Fingerprint

Chromatin Immunoprecipitation
Sonication
Neoplastic Stem Cells
Antibodies
Genes
Libraries
Chromatin
Genome

Keywords

  • ChIP-seq
  • Chromatin immunoprecipitation
  • Epigenetics
  • Histone modifications
  • Myc
  • N-Myc

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Barrilleaux, B. L., Cotterman, R., & Knoepfler, P. S. (2013). Chromatin immunoprecipitation assays for Myc and N-Myc. In Methods in Molecular Biology (Vol. 1012, pp. 117-133). (Methods in Molecular Biology; Vol. 1012). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-429-6_9

Chromatin immunoprecipitation assays for Myc and N-Myc. / Barrilleaux, Bonnie L.; Cotterman, Rebecca; Knoepfler, Paul S.

Methods in Molecular Biology. Vol. 1012 Humana Press Inc., 2013. p. 117-133 (Methods in Molecular Biology; Vol. 1012).

Research output: Chapter in Book/Report/Conference proceedingChapter

Barrilleaux, BL, Cotterman, R & Knoepfler, PS 2013, Chromatin immunoprecipitation assays for Myc and N-Myc. in Methods in Molecular Biology. vol. 1012, Methods in Molecular Biology, vol. 1012, Humana Press Inc., pp. 117-133. https://doi.org/10.1007/978-1-62703-429-6_9
Barrilleaux BL, Cotterman R, Knoepfler PS. Chromatin immunoprecipitation assays for Myc and N-Myc. In Methods in Molecular Biology. Vol. 1012. Humana Press Inc. 2013. p. 117-133. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-429-6_9
Barrilleaux, Bonnie L. ; Cotterman, Rebecca ; Knoepfler, Paul S. / Chromatin immunoprecipitation assays for Myc and N-Myc. Methods in Molecular Biology. Vol. 1012 Humana Press Inc., 2013. pp. 117-133 (Methods in Molecular Biology).
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