Choosing a mouse model: Experimental biology in context-the utility and limitations of mouse models of breast cancer

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Genetically engineered mice are critical experimental models for the study of breast cancer biology. Transgenic mice, employing strong mammary epithelial promoters to drive oncogenes, develop carcinomas with phenotypes corresponding to the molecular pathway activated. Gene-targeted (knockout) mice, in which tumor suppressors are deleted, develop mammary neoplasms with phenotypes primarily including patterns seen in spontaneous mouse mammary tumors, albeit at higher rates. Improved genetic engineering, using inducible gene expression, somatic gene transduction, conditional alleles, and crossbreeding for combined/compound genetic engineering yields precise molecular models with exquisite experimental control and phenotypes with comparative pathologic validity. Mammary gland transplantation technology adds a practical and validated method for assessing biologic behavior of selected mammary tissues. Overall, the many mouse models available are a rich resource for experimental biology with phenocopies of breast cancer subtypes, and a variety of practical advantages. The challenge is matching the model to the experimental question.

Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalCold Spring Harbor perspectives in biology
Volume3
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

Theoretical Models
Breast Neoplasms
Genetic engineering
Genetic Engineering
Phenotype
Tumors
Animal Mammary Neoplasms
Breast
Genes
Genetic Hybridization
Gene Knockout Techniques
Molecular Models
Human Mammary Glands
Oncogenes
Gene expression
Knockout Mice
Transgenic Mice
Transplantation
Alleles
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{e2fed2c6157442769e8f7d8698d5ef9d,
title = "Choosing a mouse model: Experimental biology in context-the utility and limitations of mouse models of breast cancer",
abstract = "Genetically engineered mice are critical experimental models for the study of breast cancer biology. Transgenic mice, employing strong mammary epithelial promoters to drive oncogenes, develop carcinomas with phenotypes corresponding to the molecular pathway activated. Gene-targeted (knockout) mice, in which tumor suppressors are deleted, develop mammary neoplasms with phenotypes primarily including patterns seen in spontaneous mouse mammary tumors, albeit at higher rates. Improved genetic engineering, using inducible gene expression, somatic gene transduction, conditional alleles, and crossbreeding for combined/compound genetic engineering yields precise molecular models with exquisite experimental control and phenotypes with comparative pathologic validity. Mammary gland transplantation technology adds a practical and validated method for assessing biologic behavior of selected mammary tissues. Overall, the many mouse models available are a rich resource for experimental biology with phenocopies of breast cancer subtypes, and a variety of practical advantages. The challenge is matching the model to the experimental question.",
author = "Borowsky, {Alexander D}",
year = "2011",
month = "9",
doi = "10.1101/cshperspect.a009670",
language = "English (US)",
volume = "3",
pages = "1--16",
journal = "Cold Spring Harbor perspectives in biology",
issn = "1943-0264",
publisher = "Cold Spring Harbor Laboratory Press",
number = "9",

}

TY - JOUR

T1 - Choosing a mouse model

T2 - Experimental biology in context-the utility and limitations of mouse models of breast cancer

AU - Borowsky, Alexander D

PY - 2011/9

Y1 - 2011/9

N2 - Genetically engineered mice are critical experimental models for the study of breast cancer biology. Transgenic mice, employing strong mammary epithelial promoters to drive oncogenes, develop carcinomas with phenotypes corresponding to the molecular pathway activated. Gene-targeted (knockout) mice, in which tumor suppressors are deleted, develop mammary neoplasms with phenotypes primarily including patterns seen in spontaneous mouse mammary tumors, albeit at higher rates. Improved genetic engineering, using inducible gene expression, somatic gene transduction, conditional alleles, and crossbreeding for combined/compound genetic engineering yields precise molecular models with exquisite experimental control and phenotypes with comparative pathologic validity. Mammary gland transplantation technology adds a practical and validated method for assessing biologic behavior of selected mammary tissues. Overall, the many mouse models available are a rich resource for experimental biology with phenocopies of breast cancer subtypes, and a variety of practical advantages. The challenge is matching the model to the experimental question.

AB - Genetically engineered mice are critical experimental models for the study of breast cancer biology. Transgenic mice, employing strong mammary epithelial promoters to drive oncogenes, develop carcinomas with phenotypes corresponding to the molecular pathway activated. Gene-targeted (knockout) mice, in which tumor suppressors are deleted, develop mammary neoplasms with phenotypes primarily including patterns seen in spontaneous mouse mammary tumors, albeit at higher rates. Improved genetic engineering, using inducible gene expression, somatic gene transduction, conditional alleles, and crossbreeding for combined/compound genetic engineering yields precise molecular models with exquisite experimental control and phenotypes with comparative pathologic validity. Mammary gland transplantation technology adds a practical and validated method for assessing biologic behavior of selected mammary tissues. Overall, the many mouse models available are a rich resource for experimental biology with phenocopies of breast cancer subtypes, and a variety of practical advantages. The challenge is matching the model to the experimental question.

UR - http://www.scopus.com/inward/record.url?scp=80053561248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053561248&partnerID=8YFLogxK

U2 - 10.1101/cshperspect.a009670

DO - 10.1101/cshperspect.a009670

M3 - Article

C2 - 21646376

AN - SCOPUS:80053561248

VL - 3

SP - 1

EP - 16

JO - Cold Spring Harbor perspectives in biology

JF - Cold Spring Harbor perspectives in biology

SN - 1943-0264

IS - 9

ER -