Chondrocytes from different zones exhibit characteristic differences in high density culture

Jerry C. Hu, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Superficial and middle/deep zone chondrocytes were isolated from goat femoral cartilage by a zonal abrasion method. The cells were expanded 100-fold through two passages, then seeded into agarose wells to form high-density constructs through a self-assembling process. After 4 weeks in culture, the superficial zone constructs contracted into a dense cell mass, while middle/deep zone chondrocytes formed constructs with four distinct regions. Middle/deep zone chondrocytes produced 250% more glycosaminoglycans per dry weight and more collagen per dry weight than superficial zone chondrocytes. The superficial and middle/deep zone chondrocytes were found to retain characteristic differences even after 100-fold expansion, as evidenced by construct morphology and extracellular matrix content. This study uniquely demonstrated the ability of expanded superficial and middle/deep zone chondrocytes to form constructs of distinct characteristics without a scaffold. The goal of tissue engineering different zones of cartilage is to eventually replicate the specific function of each zone.

Original languageEnglish (US)
Pages (from-to)133-140
Number of pages8
JournalConnective Tissue Research
Volume47
Issue number3
DOIs
StatePublished - May 2006
Externally publishedYes

Keywords

  • Articular cartilage
  • Cartilage zones
  • Extracellular matrix
  • Self-assembling process
  • Tissue engineering

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Nephrology
  • Cell Biology
  • Orthopedics and Sports Medicine

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