Abstract
Superficial and middle/deep zone chondrocytes were isolated from goat femoral cartilage by a zonal abrasion method. The cells were expanded 100-fold through two passages, then seeded into agarose wells to form high-density constructs through a self-assembling process. After 4 weeks in culture, the superficial zone constructs contracted into a dense cell mass, while middle/deep zone chondrocytes formed constructs with four distinct regions. Middle/deep zone chondrocytes produced 250% more glycosaminoglycans per dry weight and more collagen per dry weight than superficial zone chondrocytes. The superficial and middle/deep zone chondrocytes were found to retain characteristic differences even after 100-fold expansion, as evidenced by construct morphology and extracellular matrix content. This study uniquely demonstrated the ability of expanded superficial and middle/deep zone chondrocytes to form constructs of distinct characteristics without a scaffold. The goal of tissue engineering different zones of cartilage is to eventually replicate the specific function of each zone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 133-140 |
| Number of pages | 8 |
| Journal | Connective Tissue Research |
| Volume | 47 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 2006 |
| Externally published | Yes |
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Keywords
- Articular cartilage
- Cartilage zones
- Extracellular matrix
- Self-assembling process
- Tissue engineering
ASJC Scopus subject areas
- Biochemistry
- Immunology
- Nephrology
- Cell Biology
- Orthopedics and Sports Medicine
Cite this
Chondrocytes from different zones exhibit characteristic differences in high density culture. / Hu, Jerry C.; Athanasiou, Kyriacos A.
In: Connective Tissue Research, Vol. 47, No. 3, 05.2006, p. 133-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chondrocytes from different zones exhibit characteristic differences in high density culture
AU - Hu, Jerry C.
AU - Athanasiou, Kyriacos A.
PY - 2006/5
Y1 - 2006/5
N2 - Superficial and middle/deep zone chondrocytes were isolated from goat femoral cartilage by a zonal abrasion method. The cells were expanded 100-fold through two passages, then seeded into agarose wells to form high-density constructs through a self-assembling process. After 4 weeks in culture, the superficial zone constructs contracted into a dense cell mass, while middle/deep zone chondrocytes formed constructs with four distinct regions. Middle/deep zone chondrocytes produced 250% more glycosaminoglycans per dry weight and more collagen per dry weight than superficial zone chondrocytes. The superficial and middle/deep zone chondrocytes were found to retain characteristic differences even after 100-fold expansion, as evidenced by construct morphology and extracellular matrix content. This study uniquely demonstrated the ability of expanded superficial and middle/deep zone chondrocytes to form constructs of distinct characteristics without a scaffold. The goal of tissue engineering different zones of cartilage is to eventually replicate the specific function of each zone.
AB - Superficial and middle/deep zone chondrocytes were isolated from goat femoral cartilage by a zonal abrasion method. The cells were expanded 100-fold through two passages, then seeded into agarose wells to form high-density constructs through a self-assembling process. After 4 weeks in culture, the superficial zone constructs contracted into a dense cell mass, while middle/deep zone chondrocytes formed constructs with four distinct regions. Middle/deep zone chondrocytes produced 250% more glycosaminoglycans per dry weight and more collagen per dry weight than superficial zone chondrocytes. The superficial and middle/deep zone chondrocytes were found to retain characteristic differences even after 100-fold expansion, as evidenced by construct morphology and extracellular matrix content. This study uniquely demonstrated the ability of expanded superficial and middle/deep zone chondrocytes to form constructs of distinct characteristics without a scaffold. The goal of tissue engineering different zones of cartilage is to eventually replicate the specific function of each zone.
KW - Articular cartilage
KW - Cartilage zones
KW - Extracellular matrix
KW - Self-assembling process
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=33745000556&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745000556&partnerID=8YFLogxK
U2 - 10.1080/03008200600685392
DO - 10.1080/03008200600685392
M3 - Article
C2 - 16753806
AN - SCOPUS:33745000556
VL - 47
SP - 133
EP - 140
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 3
ER -