Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach

Galina Burdyga, Guillaume De Lartigue, Helen E Raybould, Richard Morris, Rod Dimaline, Andrea Varro, David G. Thompson, Graham J. Dockray

Research output: Contribution to journalArticle

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Abstract

The intestinal hormones CCK and PYY3-36 inhibit gastric emptying and food intake via vagal afferent neurons. Here we report that CCK regulates the expression of Y2R, at which PYY3-36 acts. In nodose ganglia from rats fasted up to 48 h, there was a fivefold decrease of Y2R mRNA compared with rats fed ad libitum; Y2R mRNA in fasted rats was increased by administration of CCK, and by refeeding through a mechanism sensitive to the CCK1R antagonist lorglumide. Antibodies to Y2R revealed expression in both neurons and satellite cells; most of the former (89 ± 4%) also expressed CCK1R. With fasting there was loss of Y2R immunoreactivity in CCK1R-expressing neurons many of which projected to the stomach, but not in satellite cells or neurons projecting to the ileum or proximal colon. Expression of a Y2R promoter-luciferase reporter (Y2R-luc) in cultured vagal afferent neurons was increased in response to CCK by 12.3 ± 0.1-fold and by phorbol ester (16.2 ± 0.4-fold); the response to both was abolished by the protein kinase C inhibitor Ro-32,0432. PYY3-36 stimulated CREB phosphorylation in rat nodose neurons after priming with CCK; in wild-type mice PYY3-36 increased Fos labeling in brainstem neurons but in mice null for CCK1R this response was abolished. Thus Y2R is expressed by functionally distinct subsets of nodose ganglion neurons projecting to the stomach and ileum/colon; in the former expression is dependent on stimulation by CCK, and there is evidence that PYY3-36 effects on vagal afferent neurons are CCK dependent.

Original languageEnglish (US)
Pages (from-to)11583-11592
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number45
DOIs
StatePublished - Nov 5 2008

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Afferent Neurons
Cholecystokinin
Stomach
Neurons
Nodose Ganglion
Ileum
Colon
Gastrointestinal Hormones
Messenger RNA
Protein C Inhibitor
Gastric Emptying
Phorbol Esters
Protein Kinase Inhibitors
Luciferases
Protein Kinase C
Brain Stem
Fasting
Eating
Phosphorylation
Antibodies

Keywords

  • CCK
  • Ghrelin
  • PYY
  • Satiety
  • Vagus
  • Y2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach. / Burdyga, Galina; De Lartigue, Guillaume; Raybould, Helen E; Morris, Richard; Dimaline, Rod; Varro, Andrea; Thompson, David G.; Dockray, Graham J.

In: Journal of Neuroscience, Vol. 28, No. 45, 05.11.2008, p. 11583-11592.

Research output: Contribution to journalArticle

Burdyga, G, De Lartigue, G, Raybould, HE, Morris, R, Dimaline, R, Varro, A, Thompson, DG & Dockray, GJ 2008, 'Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach', Journal of Neuroscience, vol. 28, no. 45, pp. 11583-11592. https://doi.org/10.1523/JNEUROSCI.2493-08.2008
Burdyga, Galina ; De Lartigue, Guillaume ; Raybould, Helen E ; Morris, Richard ; Dimaline, Rod ; Varro, Andrea ; Thompson, David G. ; Dockray, Graham J. / Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 45. pp. 11583-11592.
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