Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells

Derek D. Schramm, Janice F. Wang, Roberta R. Holt, Jodi L. Ensunsa, Jana L. Gonsalves, Sheryl A. Lazarus, Harold H. Schmitz, J. Bruce German, Carl L Keen

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Background: Polyphenolic phytochemicals inhibit vascular and inflammatory processes that contribute to disease. These effects are hypothesized to result from polyphenol-mediated alterations in cellular eicosanoid synthesis. Objective: The objective was to determine and compare the ability of cocoa procyanidins to alter eicosanoid synthesis in human subjects and cultured human aortic endothelial cells. Design: After an overnight fast, 10 healthy subjects (4 men and 6 women) consumed 37 g low-procyanidin (0.09 mg/g) and highprocyanidin (4.0 mg/g) chocolate; the treatments were separated by 1 wk. The investigation had a randomized, blinded, crossover design. Plasma samples were collected before treatment and 2 and 6 h after treatment. Eicosanoids were quantitated by enzyme immunoassay. Endothelial cells were treated in vitro with procyanidins to determine whether the effects of procyanidin in vivo were associated with procyanidin-induced alterations in endothelial cell eicosanoid synthesis. Results: Relative to the effects of the low-procyanidin chocolate, high-procyanidin chocolate induced increases in plasma prostacyclin (32%; P < 0.05) and decreases in plasma leukotrienes (29%; P < 0.04). After the in vitro procyanidin treatments, aortic endothelial cells synthesized twice as much 6-keto-prostaglandin F (P < 0.01) and 16% less leukotriene (P < 0.05) as did control cells. The in vitro and in vivo effects of procyanidins on plasma leukotriene-prostacyclin ratios in culture medium were also comparable: decreases of 58% and 52%, respectively. Conclusion: Data from this short-term investigation support the concept that certain food-derived flavonoids can favorably alter eicosanoid synthesis in humans, providing a plausible hypothesis for a mechanism by which they can decrease platelet activation in humans.

Original languageEnglish (US)
Pages (from-to)36-40
Number of pages5
JournalAmerican Journal of Clinical Nutrition
Volume73
Issue number1
StatePublished - 2001
Externally publishedYes

Fingerprint

prostacyclin
Proanthocyanidins
leukotrienes
procyanidins
eicosanoids
Leukotrienes
chocolate
Epoprostenol
Eicosanoids
endothelial cells
Endothelial Cells
synthesis
polyphenols
platelet activation
enzyme immunoassays
blood vessels
Platelet Activation
phytopharmaceuticals
prostaglandins
Phytochemicals

Keywords

  • Chocolate
  • Eicosanoids
  • Endothelial cells
  • Flavonoids
  • Leukotrienes
  • Phytochemicals

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Schramm, D. D., Wang, J. F., Holt, R. R., Ensunsa, J. L., Gonsalves, J. L., Lazarus, S. A., ... Keen, C. L. (2001). Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells. American Journal of Clinical Nutrition, 73(1), 36-40.

Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells. / Schramm, Derek D.; Wang, Janice F.; Holt, Roberta R.; Ensunsa, Jodi L.; Gonsalves, Jana L.; Lazarus, Sheryl A.; Schmitz, Harold H.; German, J. Bruce; Keen, Carl L.

In: American Journal of Clinical Nutrition, Vol. 73, No. 1, 2001, p. 36-40.

Research output: Contribution to journalArticle

Schramm, DD, Wang, JF, Holt, RR, Ensunsa, JL, Gonsalves, JL, Lazarus, SA, Schmitz, HH, German, JB & Keen, CL 2001, 'Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells', American Journal of Clinical Nutrition, vol. 73, no. 1, pp. 36-40.
Schramm, Derek D. ; Wang, Janice F. ; Holt, Roberta R. ; Ensunsa, Jodi L. ; Gonsalves, Jana L. ; Lazarus, Sheryl A. ; Schmitz, Harold H. ; German, J. Bruce ; Keen, Carl L. / Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells. In: American Journal of Clinical Nutrition. 2001 ; Vol. 73, No. 1. pp. 36-40.
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abstract = "Background: Polyphenolic phytochemicals inhibit vascular and inflammatory processes that contribute to disease. These effects are hypothesized to result from polyphenol-mediated alterations in cellular eicosanoid synthesis. Objective: The objective was to determine and compare the ability of cocoa procyanidins to alter eicosanoid synthesis in human subjects and cultured human aortic endothelial cells. Design: After an overnight fast, 10 healthy subjects (4 men and 6 women) consumed 37 g low-procyanidin (0.09 mg/g) and highprocyanidin (4.0 mg/g) chocolate; the treatments were separated by 1 wk. The investigation had a randomized, blinded, crossover design. Plasma samples were collected before treatment and 2 and 6 h after treatment. Eicosanoids were quantitated by enzyme immunoassay. Endothelial cells were treated in vitro with procyanidins to determine whether the effects of procyanidin in vivo were associated with procyanidin-induced alterations in endothelial cell eicosanoid synthesis. Results: Relative to the effects of the low-procyanidin chocolate, high-procyanidin chocolate induced increases in plasma prostacyclin (32{\%}; P < 0.05) and decreases in plasma leukotrienes (29{\%}; P < 0.04). After the in vitro procyanidin treatments, aortic endothelial cells synthesized twice as much 6-keto-prostaglandin F1α (P < 0.01) and 16{\%} less leukotriene (P < 0.05) as did control cells. The in vitro and in vivo effects of procyanidins on plasma leukotriene-prostacyclin ratios in culture medium were also comparable: decreases of 58{\%} and 52{\%}, respectively. Conclusion: Data from this short-term investigation support the concept that certain food-derived flavonoids can favorably alter eicosanoid synthesis in humans, providing a plausible hypothesis for a mechanism by which they can decrease platelet activation in humans.",
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T1 - Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells

AU - Schramm, Derek D.

AU - Wang, Janice F.

AU - Holt, Roberta R.

AU - Ensunsa, Jodi L.

AU - Gonsalves, Jana L.

AU - Lazarus, Sheryl A.

AU - Schmitz, Harold H.

AU - German, J. Bruce

AU - Keen, Carl L

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