Chlorella vulgaris production enhancement with supplementation of synthetic medium in dairy manure wastewater

Jun Shi, Pramod Pandey, Annaliese K. Franz, Huiping Deng, Richard Jeannotte

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


To identify innovative ways for better utilizing flushed dairy manure wastewater, we have assessed the effect of dairy manure and supplementation with synthetic medium on the growth of Chlorella vulgaris. A series of experiments were carried out to study the impacts of pretreatment of dairy wastewater and the benefits of supplementing dairy manure wastewater with synthetic medium on C. vulgaris growth increment and the ultrastructure (chloroplast, starch, lipid, and cell wall) of C. vulgaris cells. Results showed that the biomass production of C. vulgaris in dairy wastewater can be enhanced by pretreatment and using supplementation with synthetic media. A recipe combining pretreated dairy wastewater (40 %) and synthetic medium (60 %) exhibited an improved growth of C. vulgaris. The effects of dairy wastewater on the ultrastructure of C. vulgaris cells were distinct compared to that of cells grown in synthetic medium. The C. vulgaris growth in both synthetic medium and manure wastewater without supplementing synthetic medium was lower than the growth in dairy manure supplemented with synthetic medium. We anticipate that the results of this study will help in deriving an enhanced method of coupling nutrient-rich dairy manure wastewater for biofuel production.

Original languageEnglish (US)
Article number15
Pages (from-to)1-9
Number of pages9
JournalAMB Express
Issue number1
StatePublished - Dec 1 2016


  • Algae biomass
  • Dairy wastewater
  • Fecal bacteria
  • TEM
  • Treatment

ASJC Scopus subject areas

  • Biophysics
  • Applied Microbiology and Biotechnology
  • Orthopedics and Sports Medicine
  • Physiology (medical)


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