Chlamydia Infection Causes Loss of Pacemaker Cells and Inhibits Oocyte Transport in the Mouse Oviduct

Rose Ellen Dickson, Sung Jin Hwang, Grant W. Hennig, Kyle H. Ramsey, Justin H. Schripsema, Kenton M. Sanders, Sean M. Ward

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Chlamydia trachomatis is a common sexually transmitted bacterial infection that results in health care costs in the United States that exceed $2 billion per year. Chlamydia infections cause damage to the oviducts, resulting in ectopic pregnancy and tubal factor infertility, but the reasons for defective oviduct function are poorly understood. We have investigated the role of oviduct contractions in egg transport and found that underlying electrical pacemaker activity is responsible for oviduct motility and egg transport. Specialized pacemaker cells, referred to as oviduct interstitial cells of Cajal (ICC-OVI), are responsible for pacemaker activity. The ICC-OVI, labeled with antibodies to KIT protein, form a dense network associated with the smooth muscle cells along the entire length of the oviduct. Selective removal of ICC-OVI with KIT-neutralizing antibody resulted in loss of electrical rhythmicity and loss of propulsive contractions of the oviduct. We tested whether infection might adversely affect the ICC-OVI. Mice infected with Chlamydia muridarum displayed dilation of oviducts, pyosalpinx, and loss of spontaneous contractile activity. Morphological inspection showed disruption of ICC-OVI networks, and electrophysiological recordings showed loss of intrinsic pacemaker activity without change in basal smooth muscle membrane potential. Chlamydia infection also was associated with upregulation of NOS2 (iNOS) and PTGS2 (COX II) in leukocytes. Loss of ICC-OVI and pacemaker activity causes oviduct pseudo-obstruction and loss of propulsive contractions for oocytes. This, accompanied by retention of oviduct secretions, may contribute to the development of tubal factor infertility.

Original languageEnglish (US)
Pages (from-to)665-673
Number of pages9
JournalBiology of Reproduction
Volume80
Issue number4
DOIs
StatePublished - Apr 2009
Externally publishedYes

Keywords

  • Fallopian tubes
  • Female reproductive tract
  • Interstitial cells
  • Oviduct
  • Ovum pickup/transport

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Chlamydia Infection Causes Loss of Pacemaker Cells and Inhibits Oocyte Transport in the Mouse Oviduct'. Together they form a unique fingerprint.

  • Cite this

    Dickson, R. E., Hwang, S. J., Hennig, G. W., Ramsey, K. H., Schripsema, J. H., Sanders, K. M., & Ward, S. M. (2009). Chlamydia Infection Causes Loss of Pacemaker Cells and Inhibits Oocyte Transport in the Mouse Oviduct. Biology of Reproduction, 80(4), 665-673. https://doi.org/10.1095/biolreprod.108.073833