Chip-based wide field-of-view nanoscopy

Robin Diekmann, Øystein I. Helle, Cristina I. Øie, Peter McCourt, Thomas R Huser, Mark Schüttpelz, Balpreet S. Ahluwalia

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Present optical nanoscopy techniques use a complex microscope for imaging and a simple glass slide to hold the sample. Here, we demonstrate the inverse: the use of a complex, but mass-producible optical chip, which hosts the sample and provides a waveguide for the illumination source, and a standard low-cost microscope to acquire super-resolved images via two different approaches. Waveguides composed of a material with high refractive-index contrast provide a strong evanescent field that is used for single-molecule switching and fluorescence excitation, thus enabling chip-based single-molecule localization microscopy. Additionally, multimode interference patterns induce spatial fluorescence intensity variations that enable fluctuation-based super-resolution imaging. As chip-based nanoscopy separates the illumination and detection light paths, total-internal-reflection fluorescence excitation is possible over a large field of view, with up to 0.5 mm × 0.5 mm being demonstrated. Using multicolour chip-based nanoscopy, we visualize fenestrations in liver sinusoidal endothelial cells.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalNature Photonics
Volume11
Issue number5
DOIs
StatePublished - May 1 2017

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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    Diekmann, R., Helle, Ø. I., Øie, C. I., McCourt, P., Huser, T. R., Schüttpelz, M., & Ahluwalia, B. S. (2017). Chip-based wide field-of-view nanoscopy. Nature Photonics, 11(5), 322-328. https://doi.org/10.1038/nphoton.2017.55