Chip-based nLC-TOF-MS is a highly stable technology for large-scale high-throughput analyses

L. Renee Ruhaak, Sandra L. Taylor, Suzanne Miyamoto, Karen Kelly, Gary S Leiserowitz, David R Gandara, Carlito B Lebrilla, Kyoungmi Kim

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Many studies focused on the discovery of novel biomarkers for the diagnosis and treatment of disease states are facilitated by mass spectrometry-based technology. HPLC coupled to mass spectrometry is widely used; miniaturization of this technique using nano-liquid chromatography (LC)-mass spectrometry (MS) usually results in better sensitivity, but is associated with limited repeatability. The recent introduction of chip-based technology has significantly improved the stability of nano-LC-MS, but no substantial studies to verify this have been performed. To evaluate the temporal repeatability of chip-based nano-LCMS analyses, N-glycans released from a serum sample were repeatedly analyzed using nLC-PGC-chip-TOF-MS on three non-consecutive days. With an average inter-day coefficient of variation of 4 %, determined on log10- transformed integrals, the repeatability of the system is very high. Overall, chip-based nano-LC-MS appears to be a highly stable technology, which is suitable for the profiling of large numbers of clinical samples for biomarker discovery.

Original languageEnglish (US)
Pages (from-to)4953-4958
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number14
DOIs
StatePublished - 2013

Keywords

  • Biomarker discovery
  • N-Glycans
  • Nano-LC-MS
  • Time of flight

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Medicine(all)

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