Chinese hamster cells meet DNA repair: An entirely acceptable affair

Larry H. Thompson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

This personal account relates the advent of mutant isolation and other developments in somatic cell genetics that were critical steps toward isolating DNA repair mutants in mammalian cells. The isolation of auxotrophic and temperaturesensitive mutants in genetically stable Chinese hamster cells during the late 1960s and early 1970s provided a conceptual framework in which to later isolate mutations conferring hypersensitivity to ultraviolet radiation, ionizing radiation, and various chemical mutagens. Complementation group analysis of ultravioletsensitive mutants helped identify multiple genes that overlapped with the groups of cancer-prone xeroderma pigmentosum, as well as Cockayne syndrome. The first mammalian cell mutants defective in strand-break repair were also discovered. Subsequent cloning of human genes that corrected CHO-cell mutations in nucleotide-excision repair groups 1-6 later led to identifying the key enzymes in the incision steps of this pathway, as well as the CSB protein, which is involved in coupling excision repair and transcription.

Original languageEnglish (US)
Pages (from-to)589-597
Number of pages9
JournalBioEssays
Volume20
Issue number7
DOIs
StatePublished - Jul 1998
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Biochemistry
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

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