Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats

Etienne Pouteau, Florence Rochat, Alfred Jann, Isabelle Meirim, Jose Luis Sanchez-Garcia, Kurt Ornstein, Bruce German, Olivier Ballèvre

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0-27 litres/kg and 5.4-5.9%/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3μmol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2.The diet with chicory supplementation enhanced peripheral acetate turnover by 25% (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.

Original languageEnglish (US)
Pages (from-to)287-296
Number of pages10
JournalBritish Journal of Nutrition
Volume99
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Chicory
Butyrates
Propionates
Acetates
Food Deprivation
Diet
Inulin
Isotopes
Half-Life
Colon
Weights and Measures
Injections

Keywords

  • Chicory
  • Kinetics
  • Rats
  • Short-chain fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Pouteau, E., Rochat, F., Jann, A., Meirim, I., Sanchez-Garcia, J. L., Ornstein, K., ... Ballèvre, O. (2008). Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats. British Journal of Nutrition, 99(2), 287-296. https://doi.org/10.1017/S0007114507815790

Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats. / Pouteau, Etienne; Rochat, Florence; Jann, Alfred; Meirim, Isabelle; Sanchez-Garcia, Jose Luis; Ornstein, Kurt; German, Bruce; Ballèvre, Olivier.

In: British Journal of Nutrition, Vol. 99, No. 2, 02.2008, p. 287-296.

Research output: Contribution to journalArticle

Pouteau, E, Rochat, F, Jann, A, Meirim, I, Sanchez-Garcia, JL, Ornstein, K, German, B & Ballèvre, O 2008, 'Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats', British Journal of Nutrition, vol. 99, no. 2, pp. 287-296. https://doi.org/10.1017/S0007114507815790
Pouteau, Etienne ; Rochat, Florence ; Jann, Alfred ; Meirim, Isabelle ; Sanchez-Garcia, Jose Luis ; Ornstein, Kurt ; German, Bruce ; Ballèvre, Olivier. / Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats. In: British Journal of Nutrition. 2008 ; Vol. 99, No. 2. pp. 287-296.
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