Abstract
Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0-27 litres/kg and 5.4-5.9%/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3μmol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2.The diet with chicory supplementation enhanced peripheral acetate turnover by 25% (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 287-296 |
| Number of pages | 10 |
| Journal | British Journal of Nutrition |
| Volume | 99 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2008 |
| Externally published | Yes |
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Keywords
- Chicory
- Kinetics
- Rats
- Short-chain fatty acids
ASJC Scopus subject areas
- Medicine (miscellaneous)
Cite this
Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats. / Pouteau, Etienne; Rochat, Florence; Jann, Alfred; Meirim, Isabelle; Sanchez-Garcia, Jose Luis; Ornstein, Kurt; German, Bruce; Ballèvre, Olivier.
In: British Journal of Nutrition, Vol. 99, No. 2, 02.2008, p. 287-296.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats
AU - Pouteau, Etienne
AU - Rochat, Florence
AU - Jann, Alfred
AU - Meirim, Isabelle
AU - Sanchez-Garcia, Jose Luis
AU - Ornstein, Kurt
AU - German, Bruce
AU - Ballèvre, Olivier
PY - 2008/2
Y1 - 2008/2
N2 - Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0-27 litres/kg and 5.4-5.9%/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3μmol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2.The diet with chicory supplementation enhanced peripheral acetate turnover by 25% (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.
AB - Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0-27 litres/kg and 5.4-5.9%/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3μmol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2.The diet with chicory supplementation enhanced peripheral acetate turnover by 25% (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.
KW - Chicory
KW - Kinetics
KW - Rats
KW - Short-chain fatty acids
UR - http://www.scopus.com/inward/record.url?scp=38049097551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38049097551&partnerID=8YFLogxK
U2 - 10.1017/S0007114507815790
DO - 10.1017/S0007114507815790
M3 - Article
C2 - 17761014
AN - SCOPUS:38049097551
VL - 99
SP - 287
EP - 296
JO - British Journal of Nutrition
JF - British Journal of Nutrition
SN - 0007-1145
IS - 2
ER -