Chicken corneocyte cross-linked proteome

Robert H. Rice, Brett R. Winters, Blythe P. Durbin-Johnson, David M Rocke

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Shotgun proteomic analysis was performed of epidermal scale, feather, beak and claw from the domestic chicken. To this end, the samples were separated first into solubilized and particulate fractions, the latter enriched in isopeptide cross-linking, by exhaustive extraction in sodium dodecyl sulfate under reducing conditions. Among the 205 proteins identified were 17 keratins (types α and β), 51 involved in protein synthesis, 8 junctional, 8 histone, 5 heat shock, and 5 14-3-3 proteins. Considerable overlap among the beak, claw, feather, and scale samples was observed in protein profiles, but those from beak and claw were the most similar. Scale and feather profiles were the most distinctive, each exhibiting specific proteins. Less than 20% of the proteins were found only in the detergent-solubilized fraction, while 34-57% were found only in the particulate fraction, depending on the source, and the rest in both fractions. The results provide the first comprehensive analysis of the content of these cornified structures, reveal the efficient use of available proteins in conferring mechanical and chemical stability to them, and emphasize the importance of isopeptide cross-linking in avian epithelial cornification.

Original languageEnglish (US)
Pages (from-to)771-776
Number of pages6
JournalJournal of Proteome Research
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2013

Keywords

  • beak
  • claw
  • detergent extraction
  • epidermal scale
  • feather
  • isopeptide bonding
  • keratin
  • transglutaminase

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Rice, R. H., Winters, B. R., Durbin-Johnson, B. P., & Rocke, D. M. (2013). Chicken corneocyte cross-linked proteome. Journal of Proteome Research, 12(2), 771-776. https://doi.org/10.1021/pr301036k