Abstract
Using a purified population of rat brain astrocytes prepared from neonatal cortex, we investigated the chemotaxis of astroglia to several well characterized growth factors. Chemotactic activity for astrocytes was found for the platelet derived growth factor with a half maximal response occurring at 0.5 ng/ml as compared with a value of 2-3 ng/ml obtained for NIH/3T3 fibroblasts in control experiments. Other growth factors including epidermal growth factor, fibroblast growth factor and insulin were inactive as chemoattractants. Affinity purified fibronectin was also found to stimulate the migration of astroglia, with half maximal doses of approximately 1 μg/ml relative to maximal responses to platelet derived growth factor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 249-254 |
| Number of pages | 6 |
| Journal | Brain Research |
| Volume | 344 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 7 1985 |
| Externally published | Yes |
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Keywords
- astrocyte
- chemotaxis
- fibronectin
- platelet derived growth factor
- wound repair
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Chemotaxis of rat brain astrocytes to platelet derived growth factor. / Bressler, J. P.; Grotendorst, G. R.; Levitov, C.; Hjelmeland, Leonard M.
In: Brain Research, Vol. 344, No. 2, 07.10.1985, p. 249-254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemotaxis of rat brain astrocytes to platelet derived growth factor
AU - Bressler, J. P.
AU - Grotendorst, G. R.
AU - Levitov, C.
AU - Hjelmeland, Leonard M
PY - 1985/10/7
Y1 - 1985/10/7
N2 - Using a purified population of rat brain astrocytes prepared from neonatal cortex, we investigated the chemotaxis of astroglia to several well characterized growth factors. Chemotactic activity for astrocytes was found for the platelet derived growth factor with a half maximal response occurring at 0.5 ng/ml as compared with a value of 2-3 ng/ml obtained for NIH/3T3 fibroblasts in control experiments. Other growth factors including epidermal growth factor, fibroblast growth factor and insulin were inactive as chemoattractants. Affinity purified fibronectin was also found to stimulate the migration of astroglia, with half maximal doses of approximately 1 μg/ml relative to maximal responses to platelet derived growth factor.
AB - Using a purified population of rat brain astrocytes prepared from neonatal cortex, we investigated the chemotaxis of astroglia to several well characterized growth factors. Chemotactic activity for astrocytes was found for the platelet derived growth factor with a half maximal response occurring at 0.5 ng/ml as compared with a value of 2-3 ng/ml obtained for NIH/3T3 fibroblasts in control experiments. Other growth factors including epidermal growth factor, fibroblast growth factor and insulin were inactive as chemoattractants. Affinity purified fibronectin was also found to stimulate the migration of astroglia, with half maximal doses of approximately 1 μg/ml relative to maximal responses to platelet derived growth factor.
KW - astrocyte
KW - chemotaxis
KW - fibronectin
KW - platelet derived growth factor
KW - wound repair
UR - http://www.scopus.com/inward/record.url?scp=0022007143&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022007143&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(85)90802-9
DO - 10.1016/0006-8993(85)90802-9
M3 - Article
C2 - 3899281
AN - SCOPUS:0022007143
VL - 344
SP - 249
EP - 254
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -