Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication

Julio A. Camarero, Youngeun Kwon, Matthew A Coleman

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The present work describes a general method for the selective attachment of proteins to solid surfaces through their C-termini that can be used for the efficient creation of protein chips. Our method is based in the chemoselective reaction between a protein C-terminal α-thioester and a modified surface containing N-terminal Cys residues. α-Thioester proteins can be obtained using standard recombinant techniques by using expression vectors containing engineered inteins. This new method was used to immobilize two fluorescent proteins and a functional SH3 domain using a protein microarrayer.

Original languageEnglish (US)
Pages (from-to)14730-14731
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number45
DOIs
StatePublished - Nov 17 2004
Externally publishedYes

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Protein Array Analysis
Ligation
Membrane Proteins
Proteins
Fabrication
Inteins
src Homology Domains
Protein C

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication. / Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.

In: Journal of the American Chemical Society, Vol. 126, No. 45, 17.11.2004, p. 14730-14731.

Research output: Contribution to journalArticle

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