Chemokines and T lymphocyte activation: II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice

William J Murphy, Zhi Gang Tian, Osamu Asai, Satoshi Funakoshi, Poppy Rotter, Michelle Henry, Robert M. Strieter, Steven L. Kunkel, Dan L. Longo, Dennis D. Taub

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Previous studies from this laboratory have demonstrated that the chemokines RANTES (recombinant human regulated upon activation, normally T cell expressed and presumably secreted), macrophage chemotactic peptide-1, recombinant human macrophage inflammatory protein-1α (rhMIP-1α), IL-8, and IP-10 are capable of inducing human T cell infiltration into the injection site of severe combined immunodeficiency (SCID) mice reconstituted with human PBL. However, the ability of these chemokines to facilitate T cell homing into various lymphoid tissues has not been examined. Initial studies focused on the ability of rhMIP-1β to induce human T cell infiltration into injection sites in human PBL-SCID mice. SCID mice received s.c. injections of rhMIP-1β or PBS (1 μg/injection) in the hindflank for 4 h or sequential injections for 3 days. Biopsies of the MIP-1β injection site revealed the presence of significant mononuclear cell accumulation 72 h after injection. Immunohistologic evaluation determined that significant numbers of human CD3+ T cells were recruited in response to MIP-1β injections, and this infiltration could be specifically blocked by co-administration of anti-MIP- 1β antiserum. We subsequently examined these chemokine-injected mice for the effect of trafficking of human T cells to peripheral lymphoid organs. Flow cytometric analysis of the thymus in human PBL-SCID mice revealed that treatment with rhMIP-1β or rhRANTES, but not platelet factor-4, resulted in improved thymic homing of the human T cells after 72 h. This trafficking effect was shown to be direct, as pretreatment of the human T cells with the chemokines in vitro also improved peripheral lymphoid trafficking of the human cells. In addition, co-injection of rhMIP-1β with anti-MIP-1β antiserum abrogated the increase in T cell homing to the thymus. These data demonstrate that MIP-1β and RANTES directly augment human T cell trafficking to peripheral murine lymphoid tissues. Chemokines may, therefore, under either isogeneic or xenogeneic conditions, play a role in normal lymphocyte recirculation and homing, and may be of potential clinical use in promoting immune cell trafficking and function.

Original languageEnglish (US)
Pages (from-to)2104-2111
Number of pages8
JournalJournal of Immunology
Volume156
Issue number6
StatePublished - Mar 15 1996
Externally publishedYes

Fingerprint

Severe Combined Immunodeficiency
Lymphocyte Activation
Chemokines
T-Lymphocytes
Macrophage Inflammatory Proteins
Injections
Human Trafficking
Chemokine CCL5
Lymphoid Tissue
Thymus Gland
Immune Sera
Platelet Factor 4
Interleukin-8

ASJC Scopus subject areas

  • Immunology

Cite this

Murphy, W. J., Tian, Z. G., Asai, O., Funakoshi, S., Rotter, P., Henry, M., ... Taub, D. D. (1996). Chemokines and T lymphocyte activation: II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice. Journal of Immunology, 156(6), 2104-2111.

Chemokines and T lymphocyte activation : II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice. / Murphy, William J; Tian, Zhi Gang; Asai, Osamu; Funakoshi, Satoshi; Rotter, Poppy; Henry, Michelle; Strieter, Robert M.; Kunkel, Steven L.; Longo, Dan L.; Taub, Dennis D.

In: Journal of Immunology, Vol. 156, No. 6, 15.03.1996, p. 2104-2111.

Research output: Contribution to journalArticle

Murphy, WJ, Tian, ZG, Asai, O, Funakoshi, S, Rotter, P, Henry, M, Strieter, RM, Kunkel, SL, Longo, DL & Taub, DD 1996, 'Chemokines and T lymphocyte activation: II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice', Journal of Immunology, vol. 156, no. 6, pp. 2104-2111.
Murphy, William J ; Tian, Zhi Gang ; Asai, Osamu ; Funakoshi, Satoshi ; Rotter, Poppy ; Henry, Michelle ; Strieter, Robert M. ; Kunkel, Steven L. ; Longo, Dan L. ; Taub, Dennis D. / Chemokines and T lymphocyte activation : II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice. In: Journal of Immunology. 1996 ; Vol. 156, No. 6. pp. 2104-2111.
@article{ad8356d48ca643188da560eb5b974f84,
title = "Chemokines and T lymphocyte activation: II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice",
abstract = "Previous studies from this laboratory have demonstrated that the chemokines RANTES (recombinant human regulated upon activation, normally T cell expressed and presumably secreted), macrophage chemotactic peptide-1, recombinant human macrophage inflammatory protein-1α (rhMIP-1α), IL-8, and IP-10 are capable of inducing human T cell infiltration into the injection site of severe combined immunodeficiency (SCID) mice reconstituted with human PBL. However, the ability of these chemokines to facilitate T cell homing into various lymphoid tissues has not been examined. Initial studies focused on the ability of rhMIP-1β to induce human T cell infiltration into injection sites in human PBL-SCID mice. SCID mice received s.c. injections of rhMIP-1β or PBS (1 μg/injection) in the hindflank for 4 h or sequential injections for 3 days. Biopsies of the MIP-1β injection site revealed the presence of significant mononuclear cell accumulation 72 h after injection. Immunohistologic evaluation determined that significant numbers of human CD3+ T cells were recruited in response to MIP-1β injections, and this infiltration could be specifically blocked by co-administration of anti-MIP- 1β antiserum. We subsequently examined these chemokine-injected mice for the effect of trafficking of human T cells to peripheral lymphoid organs. Flow cytometric analysis of the thymus in human PBL-SCID mice revealed that treatment with rhMIP-1β or rhRANTES, but not platelet factor-4, resulted in improved thymic homing of the human T cells after 72 h. This trafficking effect was shown to be direct, as pretreatment of the human T cells with the chemokines in vitro also improved peripheral lymphoid trafficking of the human cells. In addition, co-injection of rhMIP-1β with anti-MIP-1β antiserum abrogated the increase in T cell homing to the thymus. These data demonstrate that MIP-1β and RANTES directly augment human T cell trafficking to peripheral murine lymphoid tissues. Chemokines may, therefore, under either isogeneic or xenogeneic conditions, play a role in normal lymphocyte recirculation and homing, and may be of potential clinical use in promoting immune cell trafficking and function.",
author = "Murphy, {William J} and Tian, {Zhi Gang} and Osamu Asai and Satoshi Funakoshi and Poppy Rotter and Michelle Henry and Strieter, {Robert M.} and Kunkel, {Steven L.} and Longo, {Dan L.} and Taub, {Dennis D.}",
year = "1996",
month = "3",
day = "15",
language = "English (US)",
volume = "156",
pages = "2104--2111",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Chemokines and T lymphocyte activation

T2 - II. Facilitation of human T cell trafficking in severe combined immunodeficiency mice

AU - Murphy, William J

AU - Tian, Zhi Gang

AU - Asai, Osamu

AU - Funakoshi, Satoshi

AU - Rotter, Poppy

AU - Henry, Michelle

AU - Strieter, Robert M.

AU - Kunkel, Steven L.

AU - Longo, Dan L.

AU - Taub, Dennis D.

PY - 1996/3/15

Y1 - 1996/3/15

N2 - Previous studies from this laboratory have demonstrated that the chemokines RANTES (recombinant human regulated upon activation, normally T cell expressed and presumably secreted), macrophage chemotactic peptide-1, recombinant human macrophage inflammatory protein-1α (rhMIP-1α), IL-8, and IP-10 are capable of inducing human T cell infiltration into the injection site of severe combined immunodeficiency (SCID) mice reconstituted with human PBL. However, the ability of these chemokines to facilitate T cell homing into various lymphoid tissues has not been examined. Initial studies focused on the ability of rhMIP-1β to induce human T cell infiltration into injection sites in human PBL-SCID mice. SCID mice received s.c. injections of rhMIP-1β or PBS (1 μg/injection) in the hindflank for 4 h or sequential injections for 3 days. Biopsies of the MIP-1β injection site revealed the presence of significant mononuclear cell accumulation 72 h after injection. Immunohistologic evaluation determined that significant numbers of human CD3+ T cells were recruited in response to MIP-1β injections, and this infiltration could be specifically blocked by co-administration of anti-MIP- 1β antiserum. We subsequently examined these chemokine-injected mice for the effect of trafficking of human T cells to peripheral lymphoid organs. Flow cytometric analysis of the thymus in human PBL-SCID mice revealed that treatment with rhMIP-1β or rhRANTES, but not platelet factor-4, resulted in improved thymic homing of the human T cells after 72 h. This trafficking effect was shown to be direct, as pretreatment of the human T cells with the chemokines in vitro also improved peripheral lymphoid trafficking of the human cells. In addition, co-injection of rhMIP-1β with anti-MIP-1β antiserum abrogated the increase in T cell homing to the thymus. These data demonstrate that MIP-1β and RANTES directly augment human T cell trafficking to peripheral murine lymphoid tissues. Chemokines may, therefore, under either isogeneic or xenogeneic conditions, play a role in normal lymphocyte recirculation and homing, and may be of potential clinical use in promoting immune cell trafficking and function.

AB - Previous studies from this laboratory have demonstrated that the chemokines RANTES (recombinant human regulated upon activation, normally T cell expressed and presumably secreted), macrophage chemotactic peptide-1, recombinant human macrophage inflammatory protein-1α (rhMIP-1α), IL-8, and IP-10 are capable of inducing human T cell infiltration into the injection site of severe combined immunodeficiency (SCID) mice reconstituted with human PBL. However, the ability of these chemokines to facilitate T cell homing into various lymphoid tissues has not been examined. Initial studies focused on the ability of rhMIP-1β to induce human T cell infiltration into injection sites in human PBL-SCID mice. SCID mice received s.c. injections of rhMIP-1β or PBS (1 μg/injection) in the hindflank for 4 h or sequential injections for 3 days. Biopsies of the MIP-1β injection site revealed the presence of significant mononuclear cell accumulation 72 h after injection. Immunohistologic evaluation determined that significant numbers of human CD3+ T cells were recruited in response to MIP-1β injections, and this infiltration could be specifically blocked by co-administration of anti-MIP- 1β antiserum. We subsequently examined these chemokine-injected mice for the effect of trafficking of human T cells to peripheral lymphoid organs. Flow cytometric analysis of the thymus in human PBL-SCID mice revealed that treatment with rhMIP-1β or rhRANTES, but not platelet factor-4, resulted in improved thymic homing of the human T cells after 72 h. This trafficking effect was shown to be direct, as pretreatment of the human T cells with the chemokines in vitro also improved peripheral lymphoid trafficking of the human cells. In addition, co-injection of rhMIP-1β with anti-MIP-1β antiserum abrogated the increase in T cell homing to the thymus. These data demonstrate that MIP-1β and RANTES directly augment human T cell trafficking to peripheral murine lymphoid tissues. Chemokines may, therefore, under either isogeneic or xenogeneic conditions, play a role in normal lymphocyte recirculation and homing, and may be of potential clinical use in promoting immune cell trafficking and function.

UR - http://www.scopus.com/inward/record.url?scp=13344276575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13344276575&partnerID=8YFLogxK

M3 - Article

C2 - 8690898

AN - SCOPUS:13344276575

VL - 156

SP - 2104

EP - 2111

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -