Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates

Candice C. Clay, Denise S.S. Rodrigues, Laurie L. Brignolo, Abbie Spinner, Ross P. Tarara, Charles Plopper, Christian M. Leutenegger, Ursula Esser

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

T-lymphocyte migratory circuits in human and nonhuman primates remain largely unexplored due to the difficulty of defining cell trafficking in vivo. However, this knowledge may reveal critical aspects of immunity and T-lymphocyte homeostasis in both health and disease. Furthermore, in vivo T-lymphocyte trafficking studies may facilitate defining mechanism(s) of immune dysfunction in the nonhuman primate model for acquired immunodeficiency syndrome (AIDS). Here, we developed a model for in vivo T-lymphocyte trafficking in nonhuman primates, and delineated homing characteristics of unstimulated peripheral blood mononuclear cells (PBMCs) to lymphoid and nonlymphoid compartments in healthy rhesus macaques. T-lymphocyte homing of autologous, carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled PBMCs was defined within 48 h of intravenous transfer. The highest relative frequency of CFSE + T lymphocytes was observed in peripheral blood and spleen. Expression of chemokine receptor CCR7 and its ligands correlated with recirculation of T lymphocytes through the periphery and homing to paracortical regions of lymph node, where cells remained largely excluded from B-cell follicles. T-lymphocyte trafficking was also detected to the liver and bone marrow, andf at low levels to the thymus and small intestine. The liver contained the highest proportion of CD45RA - T lymphocytes, consistent with homing of activated/memory T lymphocytes to this nonlymphoid site. Our data suggest that lymphoid and nonlymphoid organs are under continuous immunosurveillance in healthy macaques, and that this model may serve to investigate aberrant patterns in disease.

Original languageEnglish (US)
Pages (from-to)23-42
Number of pages20
JournalJournal of Immunological Methods
Volume293
Issue number1-2
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

Chemokines
Primates
T-Lymphocytes
Blood Cells
Immunologic Monitoring
Chemokine Receptors
Liver
Macaca
Macaca mulatta
Thymus Gland
Small Intestine
Immunity
Acquired Immunodeficiency Syndrome
Homeostasis
B-Lymphocytes
Spleen
Lymph Nodes
Bone Marrow
Ligands
Health

Keywords

  • AIDS
  • CCR7
  • CFSE
  • Homing
  • Rhesus macaque

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Clay, C. C., Rodrigues, D. S. S., Brignolo, L. L., Spinner, A., Tarara, R. P., Plopper, C., ... Esser, U. (2004). Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates. Journal of Immunological Methods, 293(1-2), 23-42. https://doi.org/10.1016/j.jim.2004.06.019

Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates. / Clay, Candice C.; Rodrigues, Denise S.S.; Brignolo, Laurie L.; Spinner, Abbie; Tarara, Ross P.; Plopper, Charles; Leutenegger, Christian M.; Esser, Ursula.

In: Journal of Immunological Methods, Vol. 293, No. 1-2, 01.10.2004, p. 23-42.

Research output: Contribution to journalArticle

Clay, CC, Rodrigues, DSS, Brignolo, LL, Spinner, A, Tarara, RP, Plopper, C, Leutenegger, CM & Esser, U 2004, 'Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates', Journal of Immunological Methods, vol. 293, no. 1-2, pp. 23-42. https://doi.org/10.1016/j.jim.2004.06.019
Clay, Candice C. ; Rodrigues, Denise S.S. ; Brignolo, Laurie L. ; Spinner, Abbie ; Tarara, Ross P. ; Plopper, Charles ; Leutenegger, Christian M. ; Esser, Ursula. / Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates. In: Journal of Immunological Methods. 2004 ; Vol. 293, No. 1-2. pp. 23-42.
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