Abstract
T-lymphocyte migratory circuits in human and nonhuman primates remain largely unexplored due to the difficulty of defining cell trafficking in vivo. However, this knowledge may reveal critical aspects of immunity and T-lymphocyte homeostasis in both health and disease. Furthermore, in vivo T-lymphocyte trafficking studies may facilitate defining mechanism(s) of immune dysfunction in the nonhuman primate model for acquired immunodeficiency syndrome (AIDS). Here, we developed a model for in vivo T-lymphocyte trafficking in nonhuman primates, and delineated homing characteristics of unstimulated peripheral blood mononuclear cells (PBMCs) to lymphoid and nonlymphoid compartments in healthy rhesus macaques. T-lymphocyte homing of autologous, carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled PBMCs was defined within 48 h of intravenous transfer. The highest relative frequency of CFSE + T lymphocytes was observed in peripheral blood and spleen. Expression of chemokine receptor CCR7 and its ligands correlated with recirculation of T lymphocytes through the periphery and homing to paracortical regions of lymph node, where cells remained largely excluded from B-cell follicles. T-lymphocyte trafficking was also detected to the liver and bone marrow, andf at low levels to the thymus and small intestine. The liver contained the highest proportion of CD45RA - T lymphocytes, consistent with homing of activated/memory T lymphocytes to this nonlymphoid site. Our data suggest that lymphoid and nonlymphoid organs are under continuous immunosurveillance in healthy macaques, and that this model may serve to investigate aberrant patterns in disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 23-42 |
| Number of pages | 20 |
| Journal | Journal of Immunological Methods |
| Volume | 293 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Oct 1 2004 |
| Externally published | Yes |
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Keywords
- AIDS
- CCR7
- CFSE
- Homing
- Rhesus macaque
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates. / Clay, Candice C.; Rodrigues, Denise S.S.; Brignolo, Laurie L.; Spinner, Abbie; Tarara, Ross P.; Plopper, Charles; Leutenegger, Christian M.; Esser, Ursula.
In: Journal of Immunological Methods, Vol. 293, No. 1-2, 01.10.2004, p. 23-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates
AU - Clay, Candice C.
AU - Rodrigues, Denise S.S.
AU - Brignolo, Laurie L.
AU - Spinner, Abbie
AU - Tarara, Ross P.
AU - Plopper, Charles
AU - Leutenegger, Christian M.
AU - Esser, Ursula
PY - 2004/10/1
Y1 - 2004/10/1
N2 - T-lymphocyte migratory circuits in human and nonhuman primates remain largely unexplored due to the difficulty of defining cell trafficking in vivo. However, this knowledge may reveal critical aspects of immunity and T-lymphocyte homeostasis in both health and disease. Furthermore, in vivo T-lymphocyte trafficking studies may facilitate defining mechanism(s) of immune dysfunction in the nonhuman primate model for acquired immunodeficiency syndrome (AIDS). Here, we developed a model for in vivo T-lymphocyte trafficking in nonhuman primates, and delineated homing characteristics of unstimulated peripheral blood mononuclear cells (PBMCs) to lymphoid and nonlymphoid compartments in healthy rhesus macaques. T-lymphocyte homing of autologous, carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled PBMCs was defined within 48 h of intravenous transfer. The highest relative frequency of CFSE + T lymphocytes was observed in peripheral blood and spleen. Expression of chemokine receptor CCR7 and its ligands correlated with recirculation of T lymphocytes through the periphery and homing to paracortical regions of lymph node, where cells remained largely excluded from B-cell follicles. T-lymphocyte trafficking was also detected to the liver and bone marrow, andf at low levels to the thymus and small intestine. The liver contained the highest proportion of CD45RA - T lymphocytes, consistent with homing of activated/memory T lymphocytes to this nonlymphoid site. Our data suggest that lymphoid and nonlymphoid organs are under continuous immunosurveillance in healthy macaques, and that this model may serve to investigate aberrant patterns in disease.
AB - T-lymphocyte migratory circuits in human and nonhuman primates remain largely unexplored due to the difficulty of defining cell trafficking in vivo. However, this knowledge may reveal critical aspects of immunity and T-lymphocyte homeostasis in both health and disease. Furthermore, in vivo T-lymphocyte trafficking studies may facilitate defining mechanism(s) of immune dysfunction in the nonhuman primate model for acquired immunodeficiency syndrome (AIDS). Here, we developed a model for in vivo T-lymphocyte trafficking in nonhuman primates, and delineated homing characteristics of unstimulated peripheral blood mononuclear cells (PBMCs) to lymphoid and nonlymphoid compartments in healthy rhesus macaques. T-lymphocyte homing of autologous, carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled PBMCs was defined within 48 h of intravenous transfer. The highest relative frequency of CFSE + T lymphocytes was observed in peripheral blood and spleen. Expression of chemokine receptor CCR7 and its ligands correlated with recirculation of T lymphocytes through the periphery and homing to paracortical regions of lymph node, where cells remained largely excluded from B-cell follicles. T-lymphocyte trafficking was also detected to the liver and bone marrow, andf at low levels to the thymus and small intestine. The liver contained the highest proportion of CD45RA - T lymphocytes, consistent with homing of activated/memory T lymphocytes to this nonlymphoid site. Our data suggest that lymphoid and nonlymphoid organs are under continuous immunosurveillance in healthy macaques, and that this model may serve to investigate aberrant patterns in disease.
KW - AIDS
KW - CCR7
KW - CFSE
KW - Homing
KW - Rhesus macaque
UR - http://www.scopus.com/inward/record.url?scp=7944237314&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7944237314&partnerID=8YFLogxK
U2 - 10.1016/j.jim.2004.06.019
DO - 10.1016/j.jim.2004.06.019
M3 - Article
VL - 293
SP - 23
EP - 42
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -