Chemically homogeneous, silylated surface for effective DNA binding and hybridization

Andrea Alessandrini, Valentina De Renzi, Lorenzo Berti, Imrich Barak, Paolo Facci

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalSurface Science
Volume582
Issue number1-3
DOIs
StatePublished - May 10 2005
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Oligonucleotide immobilization
  • Quartz crystal microbalance
  • Surface chemical reaction
  • X-ray photoelectron spectroscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces

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