Chemically homogeneous, silylated surface for effective DNA binding and hybridization

Andrea Alessandrini; Valentina De Renzi; Lorenzo Berti; Imrich Barak; Paolo Facci

doi:10.1016/j.susc.2005.03.017

Chemically homogeneous, silylated surface for effective DNA binding and hybridization

Andrea Alessandrini, Valentina De Renzi, Lorenzo Berti, Imrich Barak, Paolo Facci

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.

Original language	English (US)
Pages (from-to)	202-208
Number of pages	7
Journal	Surface Science
Volume	582
Issue number	1-3
DOIs	https://doi.org/10.1016/j.susc.2005.03.017
State	Published - May 10 2005
Externally published	Yes

Keywords

Atomic force microscopy
Oligonucleotide immobilization
Quartz crystal microbalance
Surface chemical reaction
X-ray photoelectron spectroscopy

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Condensed Matter Physics
Surfaces and Interfaces

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title = "Chemically homogeneous, silylated surface for effective DNA binding and hybridization",

abstract = "We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.",

keywords = "Atomic force microscopy, Oligonucleotide immobilization, Quartz crystal microbalance, Surface chemical reaction, X-ray photoelectron spectroscopy",

author = "Andrea Alessandrini and {De Renzi}, Valentina and Lorenzo Berti and Imrich Barak and Paolo Facci",

year = "2005",

month = may,

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doi = "10.1016/j.susc.2005.03.017",

language = "English (US)",

volume = "582",

pages = "202--208",

journal = "Surface Science",

issn = "0039-6028",

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number = "1-3",

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TY - JOUR

T1 - Chemically homogeneous, silylated surface for effective DNA binding and hybridization

AU - Alessandrini, Andrea

AU - De Renzi, Valentina

AU - Berti, Lorenzo

AU - Barak, Imrich

AU - Facci, Paolo

PY - 2005/5/10

Y1 - 2005/5/10

N2 - We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.

AB - We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.

KW - Atomic force microscopy

KW - Oligonucleotide immobilization

KW - Quartz crystal microbalance

KW - Surface chemical reaction

KW - X-ray photoelectron spectroscopy

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U2 - 10.1016/j.susc.2005.03.017

DO - 10.1016/j.susc.2005.03.017

M3 - Article

AN - SCOPUS:17944373235

VL - 582

SP - 202

EP - 208

JO - Surface Science

JF - Surface Science

SN - 0039-6028

IS - 1-3

ER -

Chemically homogeneous, silylated surface for effective DNA binding and hybridization

Abstract

Keywords

ASJC Scopus subject areas

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