Abstract
We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 202-208 |
| Number of pages | 7 |
| Journal | Surface Science |
| Volume | 582 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - May 10 2005 |
| Externally published | Yes |
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Keywords
- Atomic force microscopy
- Oligonucleotide immobilization
- Quartz crystal microbalance
- Surface chemical reaction
- X-ray photoelectron spectroscopy
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Condensed Matter Physics
- Surfaces and Interfaces
Cite this
Chemically homogeneous, silylated surface for effective DNA binding and hybridization. / Alessandrini, Andrea; De Renzi, Valentina; Berti, Lorenzo; Barak, Imrich; Facci, Paolo.
In: Surface Science, Vol. 582, No. 1-3, 10.05.2005, p. 202-208.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemically homogeneous, silylated surface for effective DNA binding and hybridization
AU - Alessandrini, Andrea
AU - De Renzi, Valentina
AU - Berti, Lorenzo
AU - Barak, Imrich
AU - Facci, Paolo
PY - 2005/5/10
Y1 - 2005/5/10
N2 - We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.
AB - We report on a method for covalent immobilization of 5′-thiol- modified single strand DNA probes, onto oxygen exposing surfaces by exploiting surface derivatization by 3-mercaptopropyltrimethoxysilane and subsequent intermolecular disulfide bond formation. The various steps in the formation of the molecular edifices have been characterized by X-ray photoelectron spectroscopy, quartz crystal microbalance and atomic force microscopy under liquid. Surface reaction kinetics of thiol-modified DNA probes with thiol-bearing silanes turned out to be a second-order one, possibly due to the presence of both free thiol and S-S dimers in solution. The ability of immobilized single strand DNA to bind the complementary strand has been tested and confirmed by quartz crystal microbalance measurements. The presented DNA immobilization method appears to be applicable to any surface bearing exposed hydroxyl moieties.
KW - Atomic force microscopy
KW - Oligonucleotide immobilization
KW - Quartz crystal microbalance
KW - Surface chemical reaction
KW - X-ray photoelectron spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=17944373235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17944373235&partnerID=8YFLogxK
U2 - 10.1016/j.susc.2005.03.017
DO - 10.1016/j.susc.2005.03.017
M3 - Article
AN - SCOPUS:17944373235
VL - 582
SP - 202
EP - 208
JO - Surface Science
JF - Surface Science
SN - 0039-6028
IS - 1-3
ER -