Abstract
Aim: To design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30). Methods: Five surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye. Results: LXY30-HEV VLP was checked for its binding and entry to a breast cancer cell line and for tumor targeting in vivo to breast cancer tissue in mice. The engineered virus-like nanoparticle not only targeted cancer cells, but also appeared immune silent to native hepatitis E virus antibodies due to epitope disruption at the antibody-binding site. Conclusion: These results demonstrate the production of a theranostic capsule suitable for cancer diagnostics and therapeutics based on surface modification of a highly stable virus-like nanoparticle.
Original language | English (US) |
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Pages (from-to) | 377-390 |
Number of pages | 14 |
Journal | Nanomedicine |
Volume | 11 |
Issue number | 4 |
DOIs | |
State | Published - Feb 1 2016 |
Keywords
- cycloaddition of targeting ligand
- cysteine replacement
- hepatitis E
- multivalent ligand display
- virus-like particle
ASJC Scopus subject areas
- Materials Science(all)
- Bioengineering
- Biomedical Engineering
- Medicine (miscellaneous)
- Development