Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product

J. G. Sutcliffe, T. M. Shinnick, N. Green, Fu-Tong Liu, H. L. Niman, R. A. Lerner

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

We previously determined the nucleotide sequence of the 3′ end of Moloney leukaemia virus and discovered the potential coding region for an unknown protein, R. We now show that this region does encode a protein. A pentadecapeptide of R was chemically synthesized and antibodies raised against it. Antisera to the synthetic peptide recognize the R protein and the env precursor polyprotein in infected cells. The strategy presented here should provide a general method for accessing proteins predicted by nucleotide sequences.

Original languageEnglish (US)
Pages (from-to)801-805
Number of pages5
JournalNature
Volume287
Issue number5785
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Medicine(all)
  • General

Fingerprint Dive into the research topics of 'Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product'. Together they form a unique fingerprint.

  • Cite this

    Sutcliffe, J. G., Shinnick, T. M., Green, N., Liu, F-T., Niman, H. L., & Lerner, R. A. (1980). Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product. Nature, 287(5785), 801-805. https://doi.org/10.1038/287801a0