Abstract
Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca2+ channels (CaV1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca2+-dependent binding of calmodulin (CaM) to CaV1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaMEF12) that contains two Ca2+ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644-1665) from CaV1.2 (BMRB accession no. 27692).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-237 |
| Number of pages | 5 |
| Journal | Biomolecular NMR assignments |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 1 2019 |
Keywords
- Calmodulin
- CaV1.2
- CDI
- EF-hand
- IQ-motif
- Synaptic plasticity
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
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Salveson, I., Anderson, D. E., Hell, J. W., & Ames, J. B. (2019).
Chemical shift assignments of a calmodulin intermediate with two Ca
2+
bound in complex with the IQ-motif of voltage-gated Ca
2+
channels (Ca
V
1.2)
Biomolecular NMR assignments, 13(1), 233-237. https://doi.org/10.1007/s12104-019-09883-0