Chemical shift assignments of a calmodulin intermediate with two Ca                         
                        2+
                                                  bound in complex with the IQ-motif of voltage-gated Ca                         
                        2+
                                                  channels (Ca                         
                        V
                                                 1.2)

Ian Salveson; David E. Anderson; Johannes W Hell; James B. Ames

doi:10.1007/s12104-019-09883-0

Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2)

Ian Salveson, David E. Anderson, Johannes W Hell, James B. Ames

Pharmacology

Research output: Contribution to journal › Article

Abstract

Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca2+ channels (CaV1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca2+-dependent binding of calmodulin (CaM) to CaV1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaMEF12) that contains two Ca2+ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644-1665) from CaV1.2 (BMRB accession no. 27692).

Original language	English (US)
Pages (from-to)	233-237
Number of pages	5
Journal	Biomolecular NMR assignments
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/s12104-019-09883-0
State	Published - Apr 1 2019

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Keywords

Calmodulin
CaV1.2
CDI
EF-hand
IQ-motif
Synaptic plasticity

ASJC Scopus subject areas

Structural Biology
Biochemistry

Cite this

Salveson, I., Anderson, D. E., Hell, J. W., & Ames, J. B. (2019). Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2) Biomolecular NMR assignments, 13(1), 233-237. https://doi.org/10.1007/s12104-019-09883-0

Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2) . / Salveson, Ian; Anderson, David E.; Hell, Johannes W; Ames, James B.

In: Biomolecular NMR assignments, Vol. 13, No. 1, 01.04.2019, p. 233-237.

Research output: Contribution to journal › Article

Salveson, I, Anderson, DE, Hell, JW & Ames, JB 2019, ' Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2) ', Biomolecular NMR assignments, vol. 13, no. 1, pp. 233-237. https://doi.org/10.1007/s12104-019-09883-0

Salveson I, Anderson DE, Hell JW, Ames JB. Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2) Biomolecular NMR assignments. 2019 Apr 1;13(1):233-237. https://doi.org/10.1007/s12104-019-09883-0

Salveson, Ian ; Anderson, David E. ; Hell, Johannes W ; Ames, James B. / Chemical shift assignments of a calmodulin intermediate with two Ca ²⁺ bound in complex with the IQ-motif of voltage-gated Ca ²⁺ channels (Ca _V 1.2) In: Biomolecular NMR assignments. 2019 ; Vol. 13, No. 1. pp. 233-237.

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10.1007/s12104-019-09883-0