Chemical shift assignments of a calmodulin intermediate with two Ca 2+ bound in complex with the IQ-motif of voltage-gated Ca 2+ channels (Ca V 1.2)

Ian Salveson, David E. Anderson, Johannes W Hell, James B. Ames

Research output: Contribution to journalArticle

Abstract

Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca2+ channels (CaV1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca2+-dependent binding of calmodulin (CaM) to CaV1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaMEF12) that contains two Ca2+ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644-1665) from CaV1.2 (BMRB accession no. 27692).

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalBiomolecular NMR assignments
Volume13
Issue number1
DOIs
StatePublished - Apr 1 2019

    Fingerprint

Keywords

  • Calmodulin
  • CaV1.2
  • CDI
  • EF-hand
  • IQ-motif
  • Synaptic plasticity

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this