Chemical reactivity at an antibody binding site elicited by mechanistic design of a synthetic antigen

A. Tramontano, K. D. Janda, R. A. Lerner

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Monoaryl phosphonate esters, designated as analogs of the transition state in the hydrolysis of carboxylic esters, were synthesized and used as haptens to generate specific monoclonal antibodies. Some of these antibodies react with cognate aryl carboxylic esters to release a fluorescent alcohol. The reaction appears to be stoichiometric; however, the activity is slowly regenerated under alkaline conditions or by treatment with hydroxylamine. Specificity is rigorous for esters of p-trifluoroacetamidophenylacetic acid, demonstrating a structural correspondence with the phosphonate hapten. Saturation kinetics are observed and kinetic parameters (k(max), V(max), and K(m)) are reported. The haptenic phosphonate is a competitive inhibitor of the reaction (K(i), 35 nM); whereas the carboxylate product of ester hydrolysis is a less effective inhibitor (K(i), ca. 7500 nM). Chemical modification of side chain groups in the protein show a parital reduction in activity on acylation of lysine or nitration of tyrosine and a dramatic quenching upon modification of histidine. The evidence is discussed in terms of a mechanism in which amino acids of the antibody combining site participate in nucleophilic and/or general base catalysis. The properties of this system suggest that it is an example of enzymic transacylation where a deacylation step is not catalyzed. The possibility of deriving enzymic function from immunological specificity through this approach is advanced.

Original languageEnglish (US)
Pages (from-to)6736-6740
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number18
StatePublished - 1986
Externally publishedYes

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Antibody Binding Sites
Synthetic Vaccines
Esters
Organophosphonates
Haptens
Hydrolysis
Hydroxylamine
Acylation
Antibodies
Catalysis
Histidine
Lysine
Tyrosine
Binding Sites
Monoclonal Antibodies
Alcohols
Amino Acids
Acids
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Chemical reactivity at an antibody binding site elicited by mechanistic design of a synthetic antigen. / Tramontano, A.; Janda, K. D.; Lerner, R. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 18, 1986, p. 6736-6740.

Research output: Contribution to journalArticle

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AB - Monoaryl phosphonate esters, designated as analogs of the transition state in the hydrolysis of carboxylic esters, were synthesized and used as haptens to generate specific monoclonal antibodies. Some of these antibodies react with cognate aryl carboxylic esters to release a fluorescent alcohol. The reaction appears to be stoichiometric; however, the activity is slowly regenerated under alkaline conditions or by treatment with hydroxylamine. Specificity is rigorous for esters of p-trifluoroacetamidophenylacetic acid, demonstrating a structural correspondence with the phosphonate hapten. Saturation kinetics are observed and kinetic parameters (k(max), V(max), and K(m)) are reported. The haptenic phosphonate is a competitive inhibitor of the reaction (K(i), 35 nM); whereas the carboxylate product of ester hydrolysis is a less effective inhibitor (K(i), ca. 7500 nM). Chemical modification of side chain groups in the protein show a parital reduction in activity on acylation of lysine or nitration of tyrosine and a dramatic quenching upon modification of histidine. The evidence is discussed in terms of a mechanism in which amino acids of the antibody combining site participate in nucleophilic and/or general base catalysis. The properties of this system suggest that it is an example of enzymic transacylation where a deacylation step is not catalyzed. The possibility of deriving enzymic function from immunological specificity through this approach is advanced.

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