Chemical changes during the early phase of in vitro oxidative damage to human LDL

G. J. Handelman, E. N. Frankel, R. Fenz, J. B. German

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Much of the oxidative damage to human LDL in vivo may lead to only minimal changes in the chemical properties of the LDL. Therefore, chemical changes were evaluated during the initial 3 hours of oxidative attack on human LDL with 5 μM Cu. HPLC analyses were calibrated with a conjugated-diene internal standard. Cholesterol-linoleate-hydroperoxide (Chol-18:2-OOH) accumulated much more rapidly than α-tocopherol was lost. Although large amounts of cholesterol arachidonate were destroyed, diene-containing oxidation products of this lipid were not identified by HPLC analysis. Phospatidyl-choline-hydroperoxides accumulated much more slowly than chol-18:2-OOH. β-carotene was oxidized relatively slowly, but lycopene was destroyed almost as fast as a-tocopherol. The preferential accumulation of chol-18:2-OOH is consistent with a model in which a-tocopherol is localized to the surface of the LDL particle, providing minimal protection to hydrophobic components in the core of the LDL.

Original languageEnglish (US)
Pages (from-to)777-788
Number of pages12
JournalBiochemistry and Molecular Biology International
Issue number4
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology


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