Chemical and biological sensing through optical resonances in microcavities

Melikhan Tanyeri, Mikaela Nichkova, Bruce D. Hammock, Ian M. Kennedy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsD.V. Nicolau, J. Enderlein, R.C. Leif, D.L. Farkas, R. Raghavachari
Pages227-236
Number of pages10
Volume5699
DOIs
StatePublished - 2005
EventImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States
Duration: Jan 24 2005Jan 27 2005

Other

OtherImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
CountryUnited States
CitySan Jose, CA
Period1/24/051/27/05

Keywords

  • Fluorescence
  • FRET
  • MDRs
  • Microdroplet
  • Quenching

ASJC Scopus subject areas

  • Engineering(all)

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