Chemical and biological sensing through optical resonances in microcavities

Melikhan Tanyeri; Mikaela Nichkova; Bruce D. Hammock; Ian M. Kennedy

doi:10.1117/12.588169

Chemical and biological sensing through optical resonances in microcavities

Melikhan Tanyeri, Mikaela Nichkova, Bruce D. Hammock, Ian M. Kennedy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Editors	D.V. Nicolau, J. Enderlein, R.C. Leif, D.L. Farkas, R. Raghavachari
Pages	227-236
Number of pages	10
Volume	5699
DOIs	https://doi.org/10.1117/12.588169
State	Published - 2005
Event	Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States Duration: Jan 24 2005 → Jan 27 2005

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Country/Territory	United States
City	San Jose, CA
Period	1/24/05 → 1/27/05

Keywords

Fluorescence
FRET
MDRs
Microdroplet
Quenching

ASJC Scopus subject areas

Engineering(all)

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10.1117/12.588169

Cite this

Chemical and biological sensing through optical resonances in microcavities. / Tanyeri, Melikhan; Nichkova, Mikaela; Hammock, Bruce D.; Kennedy, Ian M.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / D.V. Nicolau; J. Enderlein; R.C. Leif; D.L. Farkas; R. Raghavachari. Vol. 5699 2005. p. 227-236 35.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tanyeri, M, Nichkova, M, Hammock, BD & Kennedy, IM 2005, Chemical and biological sensing through optical resonances in microcavities. in DV Nicolau, J Enderlein, RC Leif, DL Farkas & R Raghavachari (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5699, 35, pp. 227-236, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, San Jose, CA, United States, 1/24/05. https://doi.org/10.1117/12.588169

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abstract = "A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.",

keywords = "Fluorescence, FRET, MDRs, Microdroplet, Quenching",

author = "Melikhan Tanyeri and Mikaela Nichkova and Hammock, {Bruce D.} and Kennedy, {Ian M.}",

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AU - Tanyeri, Melikhan

AU - Nichkova, Mikaela

AU - Hammock, Bruce D.

AU - Kennedy, Ian M.

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N2 - A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.

AB - A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.

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KW - FRET

KW - MDRs

KW - Microdroplet

KW - Quenching

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BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

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ER -

Chemical and biological sensing through optical resonances in microcavities

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