Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry

Kristen S. Kulp, Elena S F German, Mark G. Knize, David L. Shattuck, Erik J. Nelson, Ligang Wu, Jennifer L. Montgomery, James S. Felton, Kuang Jen Wu

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

We use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to image and classify individual cells on the basis of their characteristic mass spectra. Using statistical data reduction on the large data sets generated during TOF-SIMS analysis, similar biological materials can be differentiated on the basis of a combination of small changes in protein expression, metabolic activity and cell structure. We apply this powerful technique to image and differentiate three carcinoma-derived human breast cancer cell lines (MCF-7, T47D, and MDA-MB-231). In homogenized cells, we show the ability to differentiate the cell types as well as cellular compartments (cytosol, nuclear, and membrane). These studies illustrate the capacity of TOF-SIMS to characterize individual cells by chemical composition, which could ultimately be applied to detect and identify single aberrant cells within a normal cell population. Ultimately, we anticipate characterizing rare chemical changes that may provide clues to single cell progression within carcinogenic and metastatic pathways.

Original languageEnglish (US)
Pages (from-to)3651-3658
Number of pages8
JournalAnalytical Chemistry
Volume78
Issue number11
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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    Kulp, K. S., German, E. S. F., Knize, M. G., Shattuck, D. L., Nelson, E. J., Wu, L., Montgomery, J. L., Felton, J. S., & Wu, K. J. (2006). Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry. Analytical Chemistry, 78(11), 3651-3658. https://doi.org/10.1021/ac060054c