Abstract
The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a chemically specific, label-free diagnostic technique, is rapidly gaining acceptance as a powerful alternative. It has the ability to probe the chemical composition of biological materials in a non-destructive and mostly non-perturbing manner. We review the most recent developments in Raman spectroscopy in the life sciences, detailing advances in technology that have improved the ability to screen for diseases. Its role in the monitoring of biological function and mapping the cellular chemical microenvironment will be discussed. Applications including endoscopy, surface-enhanced Raman scattering (SERS), and coherent Raman scattering (CRS) will be reviewed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-73 |
| Number of pages | 11 |
| Journal | Current Opinion in Biotechnology |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2009 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans. / Wachsmann-Hogiu, Sebastian; Weeks, Tyler; Huser, Thomas R.
In: Current Opinion in Biotechnology, Vol. 20, No. 1, 02.2009, p. 63-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical analysis in vivo and in vitro by Raman spectroscopy-from single cells to humans
AU - Wachsmann-Hogiu, Sebastian
AU - Weeks, Tyler
AU - Huser, Thomas R
PY - 2009/2
Y1 - 2009/2
N2 - The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a chemically specific, label-free diagnostic technique, is rapidly gaining acceptance as a powerful alternative. It has the ability to probe the chemical composition of biological materials in a non-destructive and mostly non-perturbing manner. We review the most recent developments in Raman spectroscopy in the life sciences, detailing advances in technology that have improved the ability to screen for diseases. Its role in the monitoring of biological function and mapping the cellular chemical microenvironment will be discussed. Applications including endoscopy, surface-enhanced Raman scattering (SERS), and coherent Raman scattering (CRS) will be reviewed.
AB - The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a chemically specific, label-free diagnostic technique, is rapidly gaining acceptance as a powerful alternative. It has the ability to probe the chemical composition of biological materials in a non-destructive and mostly non-perturbing manner. We review the most recent developments in Raman spectroscopy in the life sciences, detailing advances in technology that have improved the ability to screen for diseases. Its role in the monitoring of biological function and mapping the cellular chemical microenvironment will be discussed. Applications including endoscopy, surface-enhanced Raman scattering (SERS), and coherent Raman scattering (CRS) will be reviewed.
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UR - http://www.scopus.com/inward/citedby.url?scp=65449155208&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2009.02.006
DO - 10.1016/j.copbio.2009.02.006
M3 - Article
C2 - 19268566
AN - SCOPUS:65449155208
VL - 20
SP - 63
EP - 73
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
SN - 0958-1669
IS - 1
ER -