Characterizing a rat Brca2 knockout model

M. S. Cotroneo, J. D. Haag, Y. Zan, C. C. Lopez, P. Thuwajit, G. V. Petukhova, R. D. Camerini-Otero, A. Gendron-Fitzpatrick, A. E. Griep, Christopher J Murphy, R. R. Dubielzig, M. N. Gould

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Evidence exists that BRCA2 carriers may have an elevated risk of breast, ovarian, colon, prostate, and pancreatic cancer. In general, carriers are defined as individuals with protein truncating mutations within the BRCA2 gene. Many Brca2 knockout lines have been produced and characterized in the mouse. We previously produced a rat Brca2 knockout strain in which there is a nonsense mutation in exon 11 between BRC repeats 2 and 3, and a truncated protein is produced. Interestingly, while such a mutation in homozygous mice would lead to limited survival of approximately 3 months, the Brca2-/- rats are 100% viable and the vast majority live to over 1 year of age. Brca2 -/- rats show a phenotype of growth inhibition and sterility in both sexes. Aspermatogenesis in the Brca2-/- rats is due to a failure of homologous chromosome synapsis. Long-term phenotypes include underdeveloped mammary glands, cataract formation and lifespan shortening due to the development of tumors and cancers in multiple organs. The establishment of the rat Brca2 knockout model provides a means to study the role of Brca2 in increasing cancer susceptibility and inducing a novel ocular phenotype not previously associated with this gene.

Original languageEnglish (US)
Pages (from-to)1626-1635
Number of pages10
JournalOncogene
Volume26
Issue number11
DOIs
StatePublished - Mar 8 2007
Externally publishedYes

Fingerprint

Phenotype
BRCA2 Gene
Chromosome Pairing
Neoplasms
Mutation
Nonsense Codon
Human Mammary Glands
Pancreatic Neoplasms
Ovarian Neoplasms
Colonic Neoplasms
Cataract
Infertility
Exons
Prostatic Neoplasms
Proteins
Chromosomes
Breast Neoplasms
Growth
Genes

Keywords

  • Brca2
  • Carcinogenesis
  • Knockout
  • Rat
  • Tumors

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Cotroneo, M. S., Haag, J. D., Zan, Y., Lopez, C. C., Thuwajit, P., Petukhova, G. V., ... Gould, M. N. (2007). Characterizing a rat Brca2 knockout model. Oncogene, 26(11), 1626-1635. https://doi.org/10.1038/sj.onc.1209960

Characterizing a rat Brca2 knockout model. / Cotroneo, M. S.; Haag, J. D.; Zan, Y.; Lopez, C. C.; Thuwajit, P.; Petukhova, G. V.; Camerini-Otero, R. D.; Gendron-Fitzpatrick, A.; Griep, A. E.; Murphy, Christopher J; Dubielzig, R. R.; Gould, M. N.

In: Oncogene, Vol. 26, No. 11, 08.03.2007, p. 1626-1635.

Research output: Contribution to journalArticle

Cotroneo, MS, Haag, JD, Zan, Y, Lopez, CC, Thuwajit, P, Petukhova, GV, Camerini-Otero, RD, Gendron-Fitzpatrick, A, Griep, AE, Murphy, CJ, Dubielzig, RR & Gould, MN 2007, 'Characterizing a rat Brca2 knockout model', Oncogene, vol. 26, no. 11, pp. 1626-1635. https://doi.org/10.1038/sj.onc.1209960
Cotroneo MS, Haag JD, Zan Y, Lopez CC, Thuwajit P, Petukhova GV et al. Characterizing a rat Brca2 knockout model. Oncogene. 2007 Mar 8;26(11):1626-1635. https://doi.org/10.1038/sj.onc.1209960
Cotroneo, M. S. ; Haag, J. D. ; Zan, Y. ; Lopez, C. C. ; Thuwajit, P. ; Petukhova, G. V. ; Camerini-Otero, R. D. ; Gendron-Fitzpatrick, A. ; Griep, A. E. ; Murphy, Christopher J ; Dubielzig, R. R. ; Gould, M. N. / Characterizing a rat Brca2 knockout model. In: Oncogene. 2007 ; Vol. 26, No. 11. pp. 1626-1635.
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