Characterization of zebrafish PSD-95 gene family members

Martin P. Meyer, James Trimmer, Jonathan D. Gilthorpe, Stephen J. Smith

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The PSD-95 family of membrane-associated guanylate kinases (MAGUKs) are thought to act as molecular scaffolds that regulate the assembly and function of the multiprotein signaling complex found at the postsynaptic density of excitatory synapses. Genetic analysis of PSD-95 family members in the mammalian nervous system has so far been difficult, but the zebrafish is emerging as an ideal vertebrate system for studying the role of particular genes in the developing and mature nervous system. Here we describe the cloning of the zebrafish orthologs of PSD-95, PSD-93, and two isoforms of SAP-97. Using in situ hybridization analysis we show that these zebrafish MAGUKs have overlapping but distinct patterns of expression in the developing nervous system and craniofacial skeleton. Using a pan-MAGUK antibody we show that MAGUK proteins localize to neurons within the developing hindbrain, cerebellum, visual and olfactory systems, and to skin epithelial cells. In the olfactory and visual systems MAGUK proteins are expressed strongly in synaptic regions, and the onset of expression in these areas coincides with periods of synapse formation. These data are consistent with the idea that PSD-95 family members are involved in synapse assembly and function, and provide a platform for future functional studies in vivo in a highly tractable model organism.

Original languageEnglish (US)
Pages (from-to)91-105
Number of pages15
JournalJournal of Neurobiology
Volume63
Issue number2
DOIs
StatePublished - May 2005

Fingerprint

Guanylate Kinases
Zebrafish
Synapses
Nervous System
Genes
Post-Synaptic Density
Multiprotein Complexes
Rhombencephalon
Skeleton
Cerebellum
In Situ Hybridization
Vertebrates
Organism Cloning
Protein Isoforms
Proteins
Epithelial Cells
Neurons
Skin
Antibodies

Keywords

  • Development
  • MAGUK
  • PSD-95
  • Synapse
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Meyer, M. P., Trimmer, J., Gilthorpe, J. D., & Smith, S. J. (2005). Characterization of zebrafish PSD-95 gene family members. Journal of Neurobiology, 63(2), 91-105. https://doi.org/10.1002/neu.20118

Characterization of zebrafish PSD-95 gene family members. / Meyer, Martin P.; Trimmer, James; Gilthorpe, Jonathan D.; Smith, Stephen J.

In: Journal of Neurobiology, Vol. 63, No. 2, 05.2005, p. 91-105.

Research output: Contribution to journalArticle

Meyer, MP, Trimmer, J, Gilthorpe, JD & Smith, SJ 2005, 'Characterization of zebrafish PSD-95 gene family members', Journal of Neurobiology, vol. 63, no. 2, pp. 91-105. https://doi.org/10.1002/neu.20118
Meyer, Martin P. ; Trimmer, James ; Gilthorpe, Jonathan D. ; Smith, Stephen J. / Characterization of zebrafish PSD-95 gene family members. In: Journal of Neurobiology. 2005 ; Vol. 63, No. 2. pp. 91-105.
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