Characterization of vacuolar malate and K+ channels under physiological conditions

Omar Pantoja, Angela C Gelli, Eduardo Blumwald

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Patch-clamp techniques were employed to study the electrical properties of vacuoles from sugar beet (Beta vulgaris) cell suspensions at physiological concentrations of cytoplasmic Ca2+. Vacuoles exposed to K+ malate revealed the activation of instantaneous and time-dependent outward currents by positive membrane potentials. Negative potentials induced only instantaneous inward currents. The time-dependent outward currents were 10 times more selective for malate than for K+ and were completely blocked by zinc. Vacuoles exposed to KCl developed instantaneous currents when polarized to positive or negative membrane potentials. The time-dependent outward channels could serve as the route for the movement of malate into the vacuole, whereas K+ could move through the time-independent inward and outward channels.

Original languageEnglish (US)
Pages (from-to)1137-1141
Number of pages5
JournalPlant Physiology
Volume100
Issue number3
StatePublished - Nov 1992
Externally publishedYes

ASJC Scopus subject areas

  • Plant Science

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