Characterization of urinary keratin number 18 using a new assay

P. V. Rossitto, R. Chan, M. A. Strand, C. H. Miller, W. C. Baker, A. D. Deitch, Ralph W deVere White, Robert Cardiff

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The levels of the low-molecular weight keratin 18 appearing in the voided urine of 130 healthy volunteers was studied using a new immunoradiometric assay for keratin 18 (IRMAK-18). Keratin 18 was detected in 79 urines with an average level of 2.5 ± 4.7 ng./ml. The assay does not detect complex epidermal keratins or non-keratin intermediate filaments. Only 50% of the purified keratin added to urine could be recovered. The recovered keratin had the same molecular weight as the input molecules, implying that substances in the urine physically inhibited the assay rather than destroyed the keratin. The independent variables of cell count, dead cells, and protein concentration were not found to be important in evaluating the results. However, the urine concentration, as measured by creatinine, might be an important consideration for assessing the levels of keratin detected.

Original languageEnglish (US)
Pages (from-to)431-435
Number of pages5
JournalJournal of Urology
Volume140
Issue number2
StatePublished - 1988

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Keratin-18
Keratins
Urine
Molecular Weight
Immunoradiometric Assay
Intermediate Filaments
Creatinine
Healthy Volunteers
Cell Count
Proteins

ASJC Scopus subject areas

  • Urology

Cite this

Rossitto, P. V., Chan, R., Strand, M. A., Miller, C. H., Baker, W. C., Deitch, A. D., ... Cardiff, R. (1988). Characterization of urinary keratin number 18 using a new assay. Journal of Urology, 140(2), 431-435.

Characterization of urinary keratin number 18 using a new assay. / Rossitto, P. V.; Chan, R.; Strand, M. A.; Miller, C. H.; Baker, W. C.; Deitch, A. D.; deVere White, Ralph W; Cardiff, Robert.

In: Journal of Urology, Vol. 140, No. 2, 1988, p. 431-435.

Research output: Contribution to journalArticle

Rossitto, PV, Chan, R, Strand, MA, Miller, CH, Baker, WC, Deitch, AD, deVere White, RW & Cardiff, R 1988, 'Characterization of urinary keratin number 18 using a new assay', Journal of Urology, vol. 140, no. 2, pp. 431-435.
Rossitto PV, Chan R, Strand MA, Miller CH, Baker WC, Deitch AD et al. Characterization of urinary keratin number 18 using a new assay. Journal of Urology. 1988;140(2):431-435.
Rossitto, P. V. ; Chan, R. ; Strand, M. A. ; Miller, C. H. ; Baker, W. C. ; Deitch, A. D. ; deVere White, Ralph W ; Cardiff, Robert. / Characterization of urinary keratin number 18 using a new assay. In: Journal of Urology. 1988 ; Vol. 140, No. 2. pp. 431-435.
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