Characterization of type I, II, III, IV, and V collagens by time-resolved laser-induced fluorescence spectroscopy

Laura Marcu, David Cohen, Jean Michel I Maarek, Warren S. Grundfest

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Scopus citations

Abstract

The relative proportions of genetically distinct collagen types in connective tissues vary with tissue type and change during disease progression (atherosclerosis, tumor), development, wound healing, aging. This study aims to 1) characterize the spectro-temporal fluorescence emission of five different types of collagen (Type I to V) and 2) assess the ability of time-resolved laser-induced fluorescence spectroscopy to distinguish between collagen types. Fluorescence emission of commercially available purified samples was induced with nitrogen laser excitation pulses and detected with a MCP-PMT connected to a digital storage oscilloscope. The recorded time-resolved emission spectra displayed distinct fluorescence emission characteristics for each collagen type. The time domain information complemented the spectral domain intensity data for improved discrimination between different collagen types. Our results reveal that analysis of the fluorescence emission can be used to characterize different species of collagen. Also, the results suggest that time-resolved spectroscopy can be used for monitoring of connective tissue matrix composition changes due to various pathological and non-pathological conditions.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages93-101
Number of pages9
Volume3917
StatePublished - 2000
EventOptical Biopsy III - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000

Other

OtherOptical Biopsy III
CitySan Jose, CA, USA
Period1/23/001/24/00

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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