Characterization of two neuroblastoma cell lines expressing recombinant nerve growth factor receptors

Usha Rani Reddy, Gita Venkatakrishnan, Amit K. Roy, Jie Chen, Mattie Hardy, Fulvio Mavilio, Giovanni Rovera, David E Pleasure, Alonzo H. Ross

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


In earlier studies, a 75,000-dalton glycoprotein (gp75) has been identified as a component of both low- and high-affinity nerve growth factor (NGF) receptors (NGFRs). Using an amphoteric expression vector, we have introduced the cDNA encoding the human gp75 into two neuroblastoma cell lines. SHEP is a human neuroblastoma cell line that lacks most neuronal characteristics and does not express NGFRs. The transformant line SHEP/NGFR expressed a single affinity class of NGF binding sites, did not display NGF-induced up-regulation of fos oncogene expression, and did not efficiently internalize NGF. LAN5 is a neuroblastoma cell line with neuronal characteristics, including expression of neurofilament and display of short neurites. This cell line expresses a small number of high-affinity NGFRs but no detectable low-affinity sites. The transformant line LAN5/ NGFR expressed both high- and low-affinity NGFRs, displayed NGF-induced up-regulation of fos oncogene, and efficiently internalized NGF. The number of high-affinity NGF binding sites was nearly the same for LAN5 and LAN5/ NGFR, a finding suggesting that there is a limiting number of some separately coded factor or subunit that is required for high-affinity NGFRs. Because NGF induction of fos oncogene expression correlated with expression of high-affinity NGFRs, the putative second factor may also limit NGF responsiveness.

Original languageEnglish (US)
Pages (from-to)67-74
Number of pages8
JournalJournal of Neurochemistry
Issue number1
StatePublished - 1991
Externally publishedYes


  • Fos oncogene
  • Internalization
  • Nerve growth factor
  • Receptor
  • Retrovirus

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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