Characterization of two monoclonal antibodies in an immunohistochemical study of keratin 8 and 18 expression

R. Chan, B. F. Edwards, R. Hu, P. V. Rossitto, B. H. Min, J. K. Lund, Robert Cardiff

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Two monoclonal antibodies, UCD/AB 6.11 and UCD/PR 10.11, were evaluated for their patterns of immunohistochemical reactivity in a survey of paraffin-embedded human tissues by the avidin-biotin-immunoperoxidase technique. By two-dimensional immunoblotting, UCD/AB 6.11 reacts with keratin number 18 and UCD/PR 10.11 identifies both keratin numbers 8 and 18, the major keratins found in human simple epithelial cells. Both antibodies have excellent signal-to-noise ratios, and specifically react with simple epithelia, transitional epithelia, mesothelia and tumors derived from such tissues, making them superior immunological reagents. They do not react with neural, muscle, hematopoietic, connective or most epidermal tissues. However, within a given tissue or cell type, differences in the distributions of the antigens recognized by these two antibodies can be observed, raising the possibility of differential expression, modification, or masking of the keratin epitopes revealed by UCD/AB 6.11 and UCD/PR 10.11.

Original languageEnglish (US)
Pages (from-to)472-480
Number of pages9
JournalAmerican Journal of Clinical Pathology
Issue number4
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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