Characterization of the rough endoplasmic reticulum ribosome-binding activity

Jodi M. Nunnari, Deborah L. Zimmerman, Stephen C. Ogg, Peter Walter

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

Original languageEnglish (US)
Pages (from-to)638-640
Number of pages3
JournalNature
Volume352
Issue number6336
StatePublished - Aug 15 1991
Externally publishedYes

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    Nunnari, J. M., Zimmerman, D. L., Ogg, S. C., & Walter, P. (1991). Characterization of the rough endoplasmic reticulum ribosome-binding activity. Nature, 352(6336), 638-640.