Characterization of the rough endoplasmic reticulum ribosome-binding activity

Jodi M. Nunnari, Deborah L. Zimmerman, Stephen C. Ogg, Peter Walter

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

Original languageEnglish (US)
Pages (from-to)638-640
Number of pages3
Issue number6336
StatePublished - Aug 15 1991
Externally publishedYes

ASJC Scopus subject areas

  • General


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