Characterization of the rough endoplasmic reticulum ribosome-binding activity

Jodi M. Nunnari, Deborah L. Zimmerman, Stephen C. Ogg, Peter Walter

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

Original languageEnglish (US)
Pages (from-to)638-640
Number of pages3
JournalNature
Volume352
Issue number6336
StatePublished - Aug 15 1991
Externally publishedYes

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Rough Endoplasmic Reticulum
Ribosomes
Binding Sites
Proteins
Cell Membrane
Membranes

ASJC Scopus subject areas

  • General

Cite this

Nunnari, J. M., Zimmerman, D. L., Ogg, S. C., & Walter, P. (1991). Characterization of the rough endoplasmic reticulum ribosome-binding activity. Nature, 352(6336), 638-640.

Characterization of the rough endoplasmic reticulum ribosome-binding activity. / Nunnari, Jodi M.; Zimmerman, Deborah L.; Ogg, Stephen C.; Walter, Peter.

In: Nature, Vol. 352, No. 6336, 15.08.1991, p. 638-640.

Research output: Contribution to journalArticle

Nunnari, JM, Zimmerman, DL, Ogg, SC & Walter, P 1991, 'Characterization of the rough endoplasmic reticulum ribosome-binding activity', Nature, vol. 352, no. 6336, pp. 638-640.
Nunnari JM, Zimmerman DL, Ogg SC, Walter P. Characterization of the rough endoplasmic reticulum ribosome-binding activity. Nature. 1991 Aug 15;352(6336):638-640.
Nunnari, Jodi M. ; Zimmerman, Deborah L. ; Ogg, Stephen C. ; Walter, Peter. / Characterization of the rough endoplasmic reticulum ribosome-binding activity. In: Nature. 1991 ; Vol. 352, No. 6336. pp. 638-640.
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