Characterization of the rhesus cytomegalovirus US28 locus

M. E T Penfold, T. L. Schmidt, D. J. Dairaghi, Peter A Barry, T. J. Schall

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Human cytomegalovirus (CMV) US28 (and the related open reading frame [ORF] US27) are G-protein-coupled receptor homologs believed to play a role in viral pathogenesis. In vitro, US28 has been shown to bind and internalize ligands, as well as activate intracellular signaling in response to certain chemokines, and to initiate the migration of smooth muscle cells to chemokine gradients. To assess the role of US28 in vivo, we examined the rhesus model and sequenced and characterized the rhesus CMV US28 locus. We found that rhesus CMV carries five tandem homologs of US28, all widely divergent from US28 and from each other. By reverse transcription-PCR and Northern analysis, we demonstrated expression of these ORFs in infected cells. With stable cell lines expressing these ORFs, we analyzed the homolog's binding and signaling characteristics across a wide range of chemokines and found one (RhUS28.5) to have a ligand binding profile similar to that of US28. In addition, we localized US28 and the rhesus CMV homolog RhUS28.5 to the envelope of infectious virions, suggesting a role in viral entry or cell tropism.

Original languageEnglish (US)
Pages (from-to)10404-10413
Number of pages10
JournalJournal of Virology
Volume77
Issue number19
DOIs
StatePublished - Oct 2003

Fingerprint

Cytomegalovirus
chemokines
open reading frames
Chemokines
Open Reading Frames
loci
Human herpesvirus 5
tropisms
Ligands
virion
smooth muscle
myocytes
Tropism
pathogenesis
G-Protein-Coupled Receptors
reverse transcriptase polymerase chain reaction
cell lines
Virion
cells
Reverse Transcription

ASJC Scopus subject areas

  • Immunology

Cite this

Penfold, M. E. T., Schmidt, T. L., Dairaghi, D. J., Barry, P. A., & Schall, T. J. (2003). Characterization of the rhesus cytomegalovirus US28 locus. Journal of Virology, 77(19), 10404-10413. https://doi.org/10.1128/JVI.77.19.10404-10413.2003

Characterization of the rhesus cytomegalovirus US28 locus. / Penfold, M. E T; Schmidt, T. L.; Dairaghi, D. J.; Barry, Peter A; Schall, T. J.

In: Journal of Virology, Vol. 77, No. 19, 10.2003, p. 10404-10413.

Research output: Contribution to journalArticle

Penfold, MET, Schmidt, TL, Dairaghi, DJ, Barry, PA & Schall, TJ 2003, 'Characterization of the rhesus cytomegalovirus US28 locus', Journal of Virology, vol. 77, no. 19, pp. 10404-10413. https://doi.org/10.1128/JVI.77.19.10404-10413.2003
Penfold, M. E T ; Schmidt, T. L. ; Dairaghi, D. J. ; Barry, Peter A ; Schall, T. J. / Characterization of the rhesus cytomegalovirus US28 locus. In: Journal of Virology. 2003 ; Vol. 77, No. 19. pp. 10404-10413.
@article{70a75a20646a4cacad9c4c3642b621ca,
title = "Characterization of the rhesus cytomegalovirus US28 locus",
abstract = "Human cytomegalovirus (CMV) US28 (and the related open reading frame [ORF] US27) are G-protein-coupled receptor homologs believed to play a role in viral pathogenesis. In vitro, US28 has been shown to bind and internalize ligands, as well as activate intracellular signaling in response to certain chemokines, and to initiate the migration of smooth muscle cells to chemokine gradients. To assess the role of US28 in vivo, we examined the rhesus model and sequenced and characterized the rhesus CMV US28 locus. We found that rhesus CMV carries five tandem homologs of US28, all widely divergent from US28 and from each other. By reverse transcription-PCR and Northern analysis, we demonstrated expression of these ORFs in infected cells. With stable cell lines expressing these ORFs, we analyzed the homolog's binding and signaling characteristics across a wide range of chemokines and found one (RhUS28.5) to have a ligand binding profile similar to that of US28. In addition, we localized US28 and the rhesus CMV homolog RhUS28.5 to the envelope of infectious virions, suggesting a role in viral entry or cell tropism.",
author = "Penfold, {M. E T} and Schmidt, {T. L.} and Dairaghi, {D. J.} and Barry, {Peter A} and Schall, {T. J.}",
year = "2003",
month = "10",
doi = "10.1128/JVI.77.19.10404-10413.2003",
language = "English (US)",
volume = "77",
pages = "10404--10413",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "19",

}

TY - JOUR

T1 - Characterization of the rhesus cytomegalovirus US28 locus

AU - Penfold, M. E T

AU - Schmidt, T. L.

AU - Dairaghi, D. J.

AU - Barry, Peter A

AU - Schall, T. J.

PY - 2003/10

Y1 - 2003/10

N2 - Human cytomegalovirus (CMV) US28 (and the related open reading frame [ORF] US27) are G-protein-coupled receptor homologs believed to play a role in viral pathogenesis. In vitro, US28 has been shown to bind and internalize ligands, as well as activate intracellular signaling in response to certain chemokines, and to initiate the migration of smooth muscle cells to chemokine gradients. To assess the role of US28 in vivo, we examined the rhesus model and sequenced and characterized the rhesus CMV US28 locus. We found that rhesus CMV carries five tandem homologs of US28, all widely divergent from US28 and from each other. By reverse transcription-PCR and Northern analysis, we demonstrated expression of these ORFs in infected cells. With stable cell lines expressing these ORFs, we analyzed the homolog's binding and signaling characteristics across a wide range of chemokines and found one (RhUS28.5) to have a ligand binding profile similar to that of US28. In addition, we localized US28 and the rhesus CMV homolog RhUS28.5 to the envelope of infectious virions, suggesting a role in viral entry or cell tropism.

AB - Human cytomegalovirus (CMV) US28 (and the related open reading frame [ORF] US27) are G-protein-coupled receptor homologs believed to play a role in viral pathogenesis. In vitro, US28 has been shown to bind and internalize ligands, as well as activate intracellular signaling in response to certain chemokines, and to initiate the migration of smooth muscle cells to chemokine gradients. To assess the role of US28 in vivo, we examined the rhesus model and sequenced and characterized the rhesus CMV US28 locus. We found that rhesus CMV carries five tandem homologs of US28, all widely divergent from US28 and from each other. By reverse transcription-PCR and Northern analysis, we demonstrated expression of these ORFs in infected cells. With stable cell lines expressing these ORFs, we analyzed the homolog's binding and signaling characteristics across a wide range of chemokines and found one (RhUS28.5) to have a ligand binding profile similar to that of US28. In addition, we localized US28 and the rhesus CMV homolog RhUS28.5 to the envelope of infectious virions, suggesting a role in viral entry or cell tropism.

UR - http://www.scopus.com/inward/record.url?scp=0141632792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141632792&partnerID=8YFLogxK

U2 - 10.1128/JVI.77.19.10404-10413.2003

DO - 10.1128/JVI.77.19.10404-10413.2003

M3 - Article

C2 - 12970425

AN - SCOPUS:0141632792

VL - 77

SP - 10404

EP - 10413

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 19

ER -