Characterization of the mouse collectin gene locus

Jennifer Akiyama, Stanislav V. Volik, Ingrid Plajzer-Frick, Amy Prince, Haruhiko Sago, Heinz Ulrich G Weier, Jeff N. Vanderbilt, Sam Hawgood, Francis R Poulain

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Three of the four known mouse collectin genes have been mapped to chromosome 14. To further characterize the spatial relationship of these genes, a bacterial artificial chromosome (BAC) library of mouse chromosome 14 was screened using mouse surfactant protein (SP)-A and -D complementary DNAs (cDNAs). One large clone hybridized to both SP-A and SP-D cDNAs and was found by polymerase chain reaction (PCR) to contain sequences from one of the mouse mannose-binding lectin genes (Mbl1). We used Southern mapping and subcloning of overlapping restriction fragments to characterize the gene locus. Mapping was confirmed by fluorescent in situ hybridization of fiber-stretched BAC DNA and by Southern hybridization of restriction endonuclease-digested and PCR-amplified genomic DNA. We found that the SP-A, Mbl1, and SP-D genes reside contiguously within a 55-kb region. The SP-A and Mbl1 genes are in the same 5′ to 3′ orientation and 16 kb apart. The SP-D gene is in the opposite orientation to the two other collectin genes, 13 kb away from the 3′ end of the Mbl1 gene. The mouse SP-D gene had not previously been characterized. We found its size (13 kb) and organization to be similar to that of human SP-D. Exon I is untranslated. The second exon is a hybrid exon that contains signal for initiation of translation, signal peptide, N-terminal domain, and the first seven collagen triplets of the collagen-like domain of the protein. Four short exons (III through VI) encode the collagen-like domain of the protein, and exons VII and VIII the linking and the carbohydrate-recognition domains, respectively.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume21
Issue number2
StatePublished - 1999
Externally publishedYes

Fingerprint

Collectins
Pulmonary Surfactant-Associated Protein D
Genes
Pulmonary Surfactant-Associated Protein A
Exons
Chromosomes
Bacterial Artificial Chromosomes
Chromosomes, Human, Pair 14
Collagen
Polymerase chain reaction
Complementary DNA
Mannose-Binding Lectin
Polymerase Chain Reaction
DNA
DNA Restriction Enzymes
Protein Sorting Signals
Fluorescence In Situ Hybridization
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Akiyama, J., Volik, S. V., Plajzer-Frick, I., Prince, A., Sago, H., Weier, H. U. G., ... Poulain, F. R. (1999). Characterization of the mouse collectin gene locus. American Journal of Respiratory Cell and Molecular Biology, 21(2), 193-199.

Characterization of the mouse collectin gene locus. / Akiyama, Jennifer; Volik, Stanislav V.; Plajzer-Frick, Ingrid; Prince, Amy; Sago, Haruhiko; Weier, Heinz Ulrich G; Vanderbilt, Jeff N.; Hawgood, Sam; Poulain, Francis R.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 21, No. 2, 1999, p. 193-199.

Research output: Contribution to journalArticle

Akiyama, J, Volik, SV, Plajzer-Frick, I, Prince, A, Sago, H, Weier, HUG, Vanderbilt, JN, Hawgood, S & Poulain, FR 1999, 'Characterization of the mouse collectin gene locus', American Journal of Respiratory Cell and Molecular Biology, vol. 21, no. 2, pp. 193-199.
Akiyama J, Volik SV, Plajzer-Frick I, Prince A, Sago H, Weier HUG et al. Characterization of the mouse collectin gene locus. American Journal of Respiratory Cell and Molecular Biology. 1999;21(2):193-199.
Akiyama, Jennifer ; Volik, Stanislav V. ; Plajzer-Frick, Ingrid ; Prince, Amy ; Sago, Haruhiko ; Weier, Heinz Ulrich G ; Vanderbilt, Jeff N. ; Hawgood, Sam ; Poulain, Francis R. / Characterization of the mouse collectin gene locus. In: American Journal of Respiratory Cell and Molecular Biology. 1999 ; Vol. 21, No. 2. pp. 193-199.
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