Characterization of the juvenile hormone esterases during embryogenesis of the house cricket, acheta domesticus

Michael Roe, Catherine L. Crawford, Craig W. Clifford, J. P. Woodring, Thomas C. Sparks, Bruce D. Hammock

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Juvenile hormone (JH) and α-naphthyl acetate (α-NA) esterase activity was measured on a daily basis during embryogenesis of the house cricket, Acheta domesticus. In eggs dissected from the lateral oviducts and embryos through blastokinesis, there were elevated levels of nonspecific JH esterase activity. The JH esterase activity could not be resolved from the α-NA esterase activity by gel filtration chromatography and the metabolism of both substrates was inhibited equally by 0, 0-diisopropyl phosphorofluoridate (DFP). From blastokinesis through egg hatch, the JH esterase activity was maintained at relatively low levels and was resolved from the α-NA esterase activity by gel filtration. The α-NA esterase activity was inhibited by DFP while the JH esterase activity was relatively unaffected. Low JH titers in eggs must be maintained through blastokinesis for normal development. Elevated JH esterase activity in eggs during this period appears to have a functional role in the metabolism of maternal JH in the egg.

Original languageEnglish (US)
Pages (from-to)57-71
Number of pages15
JournalInvertebrate Reproduction and Development
Volume12
Issue number1
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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