Characterization of the insulin-like growth factor (IGF) axis in the serum of maternal and fetal macaques (Macaca mulatta and Macaca fascicularis)

Alice F Tarantal, S. E. Gargosky

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Abstract

The insulin-like growth factors (IGFs) are key effecters of fetal growth and development which are modulated by serum carrier proteins (IGF binding proteins [IGFBPs]). Studies were performed to evaluate the developmental profile of IGFs and IGFBPs in the macaque, an important nonhuman primate model for human development and disease, IGF-I, IGF-II, and IGFBP-3 were studied in the rhesus (Macaca mulatta) and long-tailed (Macaca fascicularis) monkey fetus and dam during the second and third trimesters of pregnancy. Serial fetal blood samples were collected by cardiocentesis every 10 days from gestational day (GD) 90-160, and at 1 month postnatal age; maternal blood samples were collected at similar timepoints. Results indicated that maternal sera IGF-I and IGF-II did not change significantly whereas fetal concentrations of serum IGF-I and IGF-II increased approximately two-fold during the second to the third trimesters. No significant differences were detected between the two species. Western-ligand blot analysis revealed predominant IGFBPs of 45-40 (IGFBP-3) and 28 kDa (IGFBP-1) in both the maternal and fetal compartments, and Western-immunoblot analysis using a specific antisera against IGFBP-3 indicated 45-40 and 28 kDa immunoreactive forms. Thus, although IGF-I, IGF-II, and IGFBP-3 remained relatively unaffected in maternal sera during this period of gestation, fetal concentrations of IGF-I, IGF-II, and IGFBP-3 increased in a developmental profile similar to the human fetus.

Original languageEnglish (US)
Pages (from-to)190-198
Number of pages9
JournalGrowth Regulation
Volume5
Issue number4
StatePublished - 1995

Fingerprint

Insulin-Like Growth Factor Binding Proteins
Macaca fascicularis
Macaca
Somatomedins
Macaca mulatta
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor II
Mothers
Insulin-Like Growth Factor I
Serum
Third Pregnancy Trimester
Fetal Development
Fetus
Western Blotting
Insulin-Like Growth Factor Binding Protein 1
Pregnancy
Human Development
Second Pregnancy Trimester
Fetal Blood
Growth and Development

Keywords

  • Fetus
  • Growth
  • IGF-I
  • IGF-II
  • IGFBP-3
  • Monkey
  • Sonography

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Characterization of the insulin-like growth factor (IGF) axis in the serum of maternal and fetal macaques (Macaca mulatta and Macaca fascicularis)",
abstract = "The insulin-like growth factors (IGFs) are key effecters of fetal growth and development which are modulated by serum carrier proteins (IGF binding proteins [IGFBPs]). Studies were performed to evaluate the developmental profile of IGFs and IGFBPs in the macaque, an important nonhuman primate model for human development and disease, IGF-I, IGF-II, and IGFBP-3 were studied in the rhesus (Macaca mulatta) and long-tailed (Macaca fascicularis) monkey fetus and dam during the second and third trimesters of pregnancy. Serial fetal blood samples were collected by cardiocentesis every 10 days from gestational day (GD) 90-160, and at 1 month postnatal age; maternal blood samples were collected at similar timepoints. Results indicated that maternal sera IGF-I and IGF-II did not change significantly whereas fetal concentrations of serum IGF-I and IGF-II increased approximately two-fold during the second to the third trimesters. No significant differences were detected between the two species. Western-ligand blot analysis revealed predominant IGFBPs of 45-40 (IGFBP-3) and 28 kDa (IGFBP-1) in both the maternal and fetal compartments, and Western-immunoblot analysis using a specific antisera against IGFBP-3 indicated 45-40 and 28 kDa immunoreactive forms. Thus, although IGF-I, IGF-II, and IGFBP-3 remained relatively unaffected in maternal sera during this period of gestation, fetal concentrations of IGF-I, IGF-II, and IGFBP-3 increased in a developmental profile similar to the human fetus.",
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AU - Gargosky, S. E.

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N2 - The insulin-like growth factors (IGFs) are key effecters of fetal growth and development which are modulated by serum carrier proteins (IGF binding proteins [IGFBPs]). Studies were performed to evaluate the developmental profile of IGFs and IGFBPs in the macaque, an important nonhuman primate model for human development and disease, IGF-I, IGF-II, and IGFBP-3 were studied in the rhesus (Macaca mulatta) and long-tailed (Macaca fascicularis) monkey fetus and dam during the second and third trimesters of pregnancy. Serial fetal blood samples were collected by cardiocentesis every 10 days from gestational day (GD) 90-160, and at 1 month postnatal age; maternal blood samples were collected at similar timepoints. Results indicated that maternal sera IGF-I and IGF-II did not change significantly whereas fetal concentrations of serum IGF-I and IGF-II increased approximately two-fold during the second to the third trimesters. No significant differences were detected between the two species. Western-ligand blot analysis revealed predominant IGFBPs of 45-40 (IGFBP-3) and 28 kDa (IGFBP-1) in both the maternal and fetal compartments, and Western-immunoblot analysis using a specific antisera against IGFBP-3 indicated 45-40 and 28 kDa immunoreactive forms. Thus, although IGF-I, IGF-II, and IGFBP-3 remained relatively unaffected in maternal sera during this period of gestation, fetal concentrations of IGF-I, IGF-II, and IGFBP-3 increased in a developmental profile similar to the human fetus.

AB - The insulin-like growth factors (IGFs) are key effecters of fetal growth and development which are modulated by serum carrier proteins (IGF binding proteins [IGFBPs]). Studies were performed to evaluate the developmental profile of IGFs and IGFBPs in the macaque, an important nonhuman primate model for human development and disease, IGF-I, IGF-II, and IGFBP-3 were studied in the rhesus (Macaca mulatta) and long-tailed (Macaca fascicularis) monkey fetus and dam during the second and third trimesters of pregnancy. Serial fetal blood samples were collected by cardiocentesis every 10 days from gestational day (GD) 90-160, and at 1 month postnatal age; maternal blood samples were collected at similar timepoints. Results indicated that maternal sera IGF-I and IGF-II did not change significantly whereas fetal concentrations of serum IGF-I and IGF-II increased approximately two-fold during the second to the third trimesters. No significant differences were detected between the two species. Western-ligand blot analysis revealed predominant IGFBPs of 45-40 (IGFBP-3) and 28 kDa (IGFBP-1) in both the maternal and fetal compartments, and Western-immunoblot analysis using a specific antisera against IGFBP-3 indicated 45-40 and 28 kDa immunoreactive forms. Thus, although IGF-I, IGF-II, and IGFBP-3 remained relatively unaffected in maternal sera during this period of gestation, fetal concentrations of IGF-I, IGF-II, and IGFBP-3 increased in a developmental profile similar to the human fetus.

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