Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs

Thierry Olivry, Stanley M. Dunston, K. Marcy Murphy, Peter F Moore

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late-phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE-mediated late-phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae-allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate-buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post-injection. Sections were stained with metachromatic and eosinophil-specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late-phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE-specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of αβ T-lymphocytes and dermal dendritic cells in later stages of the late-phase reactions. Because IgE-mediated late-phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti-inflammatory effects of anti-allergic drugs in a pre-clinical setting.

Original languageEnglish (US)
Pages (from-to)49-58
Number of pages10
JournalVeterinary Dermatology
Volume12
Issue number1
DOIs
StatePublished - Feb 2001

Fingerprint

skin (animal)
Immunoglobulin E
Canidae
Dogs
Skin
dogs
Allergens
allergens
Eosinophils
Antibodies
eosinophils
injection
Dermatophagoides farinae
antibodies
Intradermal Injections
Anti-Allergic Agents
Injections
Langerhans Cells
Mites
HLA Antigens

Keywords

  • Allergy
  • Atopic dermatitis
  • Dog
  • Late-phase reaction
  • Skin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs. / Olivry, Thierry; Dunston, Stanley M.; Murphy, K. Marcy; Moore, Peter F.

In: Veterinary Dermatology, Vol. 12, No. 1, 02.2001, p. 49-58.

Research output: Contribution to journalArticle

@article{2329ecd8e3954c668fdab852bffc2041,
title = "Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs",
abstract = "In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late-phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE-mediated late-phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae-allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate-buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post-injection. Sections were stained with metachromatic and eosinophil-specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late-phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE-specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of αβ T-lymphocytes and dermal dendritic cells in later stages of the late-phase reactions. Because IgE-mediated late-phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti-inflammatory effects of anti-allergic drugs in a pre-clinical setting.",
keywords = "Allergy, Atopic dermatitis, Dog, Late-phase reaction, Skin",
author = "Thierry Olivry and Dunston, {Stanley M.} and Murphy, {K. Marcy} and Moore, {Peter F}",
year = "2001",
month = "2",
doi = "10.1046/j.1365-3164.2001.00230.x",
language = "English (US)",
volume = "12",
pages = "49--58",
journal = "Veterinary Dermatology",
issn = "0959-4493",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs

AU - Olivry, Thierry

AU - Dunston, Stanley M.

AU - Murphy, K. Marcy

AU - Moore, Peter F

PY - 2001/2

Y1 - 2001/2

N2 - In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late-phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE-mediated late-phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae-allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate-buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post-injection. Sections were stained with metachromatic and eosinophil-specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late-phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE-specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of αβ T-lymphocytes and dermal dendritic cells in later stages of the late-phase reactions. Because IgE-mediated late-phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti-inflammatory effects of anti-allergic drugs in a pre-clinical setting.

AB - In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late-phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE-mediated late-phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae-allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate-buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post-injection. Sections were stained with metachromatic and eosinophil-specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late-phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE-specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of αβ T-lymphocytes and dermal dendritic cells in later stages of the late-phase reactions. Because IgE-mediated late-phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti-inflammatory effects of anti-allergic drugs in a pre-clinical setting.

KW - Allergy

KW - Atopic dermatitis

KW - Dog

KW - Late-phase reaction

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0035260986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035260986&partnerID=8YFLogxK

U2 - 10.1046/j.1365-3164.2001.00230.x

DO - 10.1046/j.1365-3164.2001.00230.x

M3 - Article

C2 - 11301539

AN - SCOPUS:0035260986

VL - 12

SP - 49

EP - 58

JO - Veterinary Dermatology

JF - Veterinary Dermatology

SN - 0959-4493

IS - 1

ER -