Characterization of the first Bartonella henselae strain isolated from a cat in Italy

Lorenzo Ciceroni, Massimo Fabbi, Simonetta Ciarrocchi, Antonella Pinto, Alessandra Ciervo, Rickie W. Kasten, Bruno B Chomel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bartonella henselae has been identified and characterized for the first time in Italy. A strain, designed Pavia-1, was isolated from the blood of a cat whose owner developed cat scratch disease (CSD). Pavia-1 and two American B. henselae strains (Houston-1, ATCC 49882, type I and strain 269608, UC Davis, type II) were compared by whole-cell fatty analysis (CFA), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiles, Western immunoblotting (WB) for reactivity with polyclonal antibodies, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), type-specific 16S rRNA PCRs, and pulsed-field gel electrophoresis (PFGE). Bartonella clarridgeiae (ATCC 51734) was also included for comparison. Pavia-1 was identified as a B. henselae type I. PFGE allowed differentiation between B. clarridgeiae and B. henselae and furthermore, between all the B. henselae strains. The fingerprints of PFGE observed for Pavia-1 were distinct from those of B. henselae type II and also of Houston-1, suggesting that the two type I strains derived from two different clones. These results show the capability of B. henselae to develop genotypic variability between genetically related strains.

Original languageEnglish (US)
Pages (from-to)217-228
Number of pages12
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume25
Issue number4
DOIs
StatePublished - 2002

Fingerprint

Bartonella henselae
Italy
Cats
Aesculus
cats
Pulsed Field Gel Electrophoresis
Bartonella clarridgeiae
pulsed-field gel electrophoresis
cat scratch disease
Bartonella
Cat-Scratch Disease
Polymerase Chain Reaction
Dermatoglyphics
immunoblotting
polyclonal antibodies
Sodium Dodecyl Sulfate
Restriction Fragment Length Polymorphisms
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
restriction fragment length polymorphism

Keywords

  • Bartonella henselae
  • Cat
  • Characterization
  • Italy

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

Cite this

Characterization of the first Bartonella henselae strain isolated from a cat in Italy. / Ciceroni, Lorenzo; Fabbi, Massimo; Ciarrocchi, Simonetta; Pinto, Antonella; Ciervo, Alessandra; Kasten, Rickie W.; Chomel, Bruno B.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 25, No. 4, 2002, p. 217-228.

Research output: Contribution to journalArticle

Ciceroni, Lorenzo ; Fabbi, Massimo ; Ciarrocchi, Simonetta ; Pinto, Antonella ; Ciervo, Alessandra ; Kasten, Rickie W. ; Chomel, Bruno B. / Characterization of the first Bartonella henselae strain isolated from a cat in Italy. In: Comparative Immunology, Microbiology and Infectious Diseases. 2002 ; Vol. 25, No. 4. pp. 217-228.
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